dnaD [Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)]

initiation of chromosome replication, part of the replisome

locus: BSU22350

pI: 7.17

mw: 27.00 kDa

function: DNA replication

product: initiation of chromosome replication

essential: yes

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

[SW|Categories] containing this gene/protein

• [SW|DNA replication], [SW|essential genes]

Gene

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• [protein|search|DnaD]-mediated untwisting of the DNA stimulates [protein|search|Nth] endonuclease activity at AP sites [Pubmed|21954439]
• required for the assembly of the replicative helicase DnaC at the origin of replication [Pubmed|22821970]
• interaction with DnaA inhibits the ability of DnaA to bind cooperatively to DNA [Pubmed|22821970]

Protein family

• DNA polymerase type-Y family (according to Swiss-Prot)

Structure

• [PDB|2C79] (N-terminal domain)

[SW|Localization]

• Membrane-proximal (Spotty) [Pubmed|16479537]

[SW|Interactions]

• [protein|search|DnaD]-[protein|search|PriA] [Pubmed|11585815]
• [protein|search|DnaD]-[protein|search|DnaA] [Pubmed|22821970,11222620]
• [protein|search|DnaD]-[protein|search|DnaB] [Pubmed|15186423,19707554]
• part of the [SW|replisome]: [protein|search|PolC]-[protein|search|HolA]-[protein|search|HolB]-[protein|search|DnaX]-[protein|search|DnaN]-[protein|search|DnaG]-[protein|search|DnaC]-[protein|search|DnaI]-[protein|search|DnaD]-[protein|search|SsbA]-[protein|search|DnaE]-[protein|search|PriA]-[protein|search|DnaB] [Pubmed|20122408]
• part of the [SW|primosome]: [protein|search|DnaA]-[protein|search|DnaG]-[protein|search|DnaC]-[protein|search|DnaI]-[protein|search|DnaD]-[protein|search|DnaB] [Pubmed|22797751]

Expression and Regulation

Operon

• ''[protein|search|dnaD]-[protein|search|nth]'' [Pubmed|21954439]

Regulation

• constitutively expressed [Pubmed|21954439]
• there are about 3,000 to 5,000 molecules of [protein|search|DnaD] per cell [Pubmed|15686560]
• expression is transiently increased after treatment with H2O2 [Pubmed|21954439]

Labs working on this gene/protein

• [SW|Alan Grossman], MIT, Cambridge, MA, USA

References

Reviews

• 22797751

Original publications

• 19707554,15686560,15186423,11585815,20122408,16479537,11222620,19968790,11679082,20587500,21097613,21954439,22821970