dnaD [Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)]

initiation of chromosome replication, part of the replisome

Locus: BSU22350

Isoelectric point: 7.17

Molecular weight: 27.00 kDa

Function: DNA replication

Product: initiation of chromosome replication

Essential: yes

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Genetics → DNA replication
• Groups of genes → Essential genes

Categories containing this gene/protein

• DNA replication, essential genes

Gene

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• DnaD-mediated untwisting of the DNA stimulates Nth endonuclease activity at AP sites PubMed
• required for the assembly of the replicative helicase DnaC at the origin of replication PubMed
• interaction with DnaA inhibits the ability of DnaA to bind cooperatively to DNA PubMed

Protein family

• DNA polymerase type-Y family (according to Swiss-Prot)

Structure

• 2C79 (N-terminal domain)

Localization

• Membrane-proximal (Spotty) PubMed

Interactions

• DnaD-PriA PubMed
• DnaD-DnaA PubMed
• DnaD-DnaB PubMed
• part of the replisome: PolC-HolA-HolB-DnaX-DnaN-DnaG-DnaC-DnaI-DnaD-SsbA-DnaE-PriA-DnaB PubMed
• part of the primosome: DnaA-DnaG-DnaC-DnaI-DnaD-DnaB PubMed

Expression and Regulation

Operon

• dnaD-nth PubMed

Regulation

• constitutively expressed PubMed
• there are about 3,000 to 5,000 molecules of DnaD per cell PubMed
• expression is transiently increased after treatment with H2O2 PubMed

Labs working on this gene/protein

• Alan Grossman, MIT, Cambridge, MA, USA

References

Reviews

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Original publications

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