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pucA [2019-08-09 09:55:33]
function
purine utilization
product
xanthine dehydrogenase
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.1|Utilization of nucleotides]Gene
Coordinates
3,339,945 → 3,340,937
The protein
Catalyzed reaction/ biological activity
Xanthine + NAD+ + H2O = urate + NADH (according to Swiss-Prot)Structure
[PDB|3ON5] (from B. halodurans, 44% identity)Expression and Regulation
Operons
genes
[gene|C9370B221117B89A1134B413748D39505C2E1DA8|pucA]-[gene|99428BD0B1A8E91F4B028ACF8AB51B235DDB4F11|pucB]-[gene|D0F00ACF5AAD9E9418033F5B47527C4D50007F18|pucC]-[gene|B65E4B340961332ED0ACC7FD957351256F708F60|pucD]-[gene|6855CC156FCB4C4A16AFD6ADE18F3FFC82B61843|pucE]
description
[Pubmed|12823818]
sigma factors
[protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12029039], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]regulatory mechanism
[protein|52C1601482C26400A524E880334BB801F832D6ED|PucR]: activation, [Pubmed|12029039], in [regulon|52C1601482C26400A524E880334BB801F832D6ED|PucR regulon]regulation
induced in the presence of purine nucleotides (effector: allantoin) ([protein|search|PucR]) [Pubmed|12029039]view in new tabBiological materials
Mutant
MGNA-A965 (yurF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/965 NBRP B. subtilis, Japan]BKE32510 (Δ[gene|C9370B221117B89A1134B413748D39505C2E1DA8|pucA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32510 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGCCGCATCCTCCTCTC, downstream forward: _UP4_ACAAGAAAACGGGTGGCGGTBKK32510 (Δ[gene|C9370B221117B89A1134B413748D39505C2E1DA8|pucA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32510 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATGCCGCATCCTCCTCTC, downstream forward: _UP4_ACAAGAAAACGGGTGGCGGTReferences
11344136,12029039,12823818