polY1 [2019-08-07 10:27:58]

translesion synthesis (TLS-) DNA polymerase Y1, important for stationary phase mutagenesis, promotes efficient replisome progression through lagging-strand genes, thereby reducing potentially detrimental breaks and single-stranded DNA at these loci

Locus: BSU_23870

Isoelectric point: 9.95

Molecular weight: 46.86 kDa

Protein length: 414 aa

Gene length: 1245 bp

Function: generation of mutations in stationary phase

Product: translesion synthesis (TLS-) DNA polymerase Y1

Essential: no

E.C.: 2.7.7.7

Synonyms: yqjH

Genomic Context

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Categories containing this gene/protein

• Information processing → Genetics → DNA repair/ recombination
• Information processing → Genetics → DNA repair/ recombination → Other proteins

Gene

Coordinates: 2,482,269 → 2,483,513

Phenotypes of a mutant

• reduced UV-induced mutagenesis PubMed
• sensitive to blue light-induced DNA damage PubMed
• increased sensitivity to Cr(VI) PubMed

The protein

Catalyzed reaction/ biological activity

• Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1) (according to Swiss-Prot)
• required for Cr(VI)-induced mutagenesis PubMed

Protein family

• DNA polymerase type-Y family (according to UniProt)

Paralogous protein(s)

• PolY2, UvrX

Structure

• 4IRC (from E. coli, 35% identity) PubMed

Localization

• cytoplasm (homogeneous)

Expression and Regulation

Operons

• Genes: polY1-gndA
  Description: PubMed

  Regulation

  • constitutively expressed PubMed

Biological materials

Mutant

• MGNA-C390 (yqjH::erm), available at the NBRP B. subtilis, Japan
• GP1111 (spc), available in Stülke lab
• BKE23870 (ΔpolY1::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAAACAAATCTCCTTTTC, downstream forward: _UP4_TGAATCGCTTGAAAAAAAGG
• BKK23870 (ΔpolY1::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAAACAAATCTCCTTTTC, downstream forward: _UP4_TGAATCGCTTGAAAAAAAGG

References

Reviews

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Original publications

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