polY1 [Tue Dec 13 2016 11:16:34 GMT+0100 (CET)]

translesion synthesis (TLS-) DNA polymerase Y1, important for stationary phase mutagenesis, promotes efficient replisome progression through lagging-strand genes, thereby reducing potentially detrimental breaks and single-stranded DNA at these loci

locus: BSU23870

pI: 9.95

mw: 46.00 kDa

function: generation of mutations in stationary phase

product: translesion synthesis (TLS-) DNA polymerase Y1

essential: no

ec: 2.7.7.7

synonyms: yqjH

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]

[SW|Categories] containing this gene/protein

• [SW|DNA repair/ recombination]

Gene

Coordinates on the chromosome (coding sequence): 2,482,269 -> 2,483,513

Phenotypes of a mutant

• reduced UV-induced mutagenesis [Pubmed|12644484]

The protein

Catalyzed reaction/ biological activity

• Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1) (according to Swiss-Prot)

Protein family

• DNA polymerase type-Y family (according to Swiss-Prot)

Paralogous protein(s)

• [SW|PolY2], [protein|search|UvrX], [protein|search|YozK]-[protein|search|YobH]

[SW|Localization]

• cytoplasm (homogeneous)

[SW|Interactions]

• [SW|PolY1]-[protein|search|DnaN] (required for untargeted mutagenesis) [Pubmed|15469515]
• [protein|search|PolA]-[SW|PolY1]-[protein|search|DnaN] [Pubmed|16045613]

Expression and Regulation

Operon

• ''[SW|polY1]-[protein|search|gndA]'' (according to [http://dbtbs.hgc.jp/COG/prom/yqjHI.html DBTBS])

Regulation

• constitutively expressed [Pubmed|15469515]

Biological materials

Mutant

• GP1111 (spc), available in [SW|Stülke] lab

References

Reviews

• 22933559

Original publications

• 12644484,15469515,16045613,19924481,23686288,25713353