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pgm [Mon Aug 31 2015 13:56:52 GMT+0200 (CEST)]
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pgm [Mon Aug 31 2015 13:56:52 GMT+0200 (CEST)]

phosphoglycerate mutase, glycolytic / gluconeogenic enzyme
locus
BSU33910
pI
5.21
mw
56.10 kDa
function
enzyme in glycolysis / gluconeogenesis
product
2,3-bisphosphoglycerate-independent
phosphoglycerate mutase
essential
Yes
ec
5.4.2.1
synonyms
gpmI
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.1|Glycolysis]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.2|Gluconeogenesis]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]
    • syns
    gpmI

    [SW|Categories] containing this gene/protein

  • [SW|carbon core metabolism], [SW|essential genes], [SW|phosphoproteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|CggR regulon]

    • Gene

    Phenotypes of a mutant

  • Essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • 2-phospho-D-glycerate = 3-phospho-D-glycerate (according to Swiss-Prot)

    • Protein family

  • BPG-independent phosphoglycerate mutase family (according to Swiss-Prot)

    • Kinetic information

  • Reversible Michaelis-Menten [Pubmed|33963]

    • Modification

  • phosphorylation on Ser-62 [Pubmed|17218307], [Pubmed|17726680]

    • [SW|Cofactors]

  • Mn2+

    • Effectors of protein activity

  • Inhibited by diverse divalent heavy-metal ions, EDTA and 2,3-butanedione [Pubmed|33963]
  • 2,3-Diphosphoglycerate has NO role on this enzyme regulation [Pubmed|33963]

    • Structure

  • [PDB|1EJJ] (Geobacillus stearothermophilus, complex with 3-phosphoglycerate), [PDB|1EQJ] (Geobacillus stearothermophilus, complex with 2-phosphoglycerate), ''Geobacillus stearothermophilus'', complex with 2-phosphoglycerate [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=16359 NCBI], ''Geobacillus stearothermophilus'', complex with 3-phosphoglycerate [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=15578 NCBI]

    • [SW|Localization]

  • Cytoplasm (Homogeneous) [Pubmed|16479537]

    • [SW|Interactions]

  • Pgm-[protein|search|PfkA]

    • Additional information

  • extensive information on the structure and enzymatic properties of Pgm can be found at [http://www.proteopedia.org/wiki/index.php/Phosphoglycerate_Mutase Proteopedia]

    • Expression and Regulation

    Operon

  • ''[protein|search|cggR]-[protein|search|gapA]-[protein|search|pgk]-[protein|search|tpiA]-[protein|search|pgm]-[protein|search|eno]'' [Pubmed|11489127]
  • ''[protein|search|pgk]-[protein|search|tpiA]-[protein|search|pgm]-[protein|search|eno]'' [Pubmed|11489127]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|11489127]

    • Regulation

  • expression activated by glucose (3.3 fold) [Pubmed|12850135]
  • ''[protein|search|cggR]'': induced by glycolytic substrates [protein|search|CggR] [Pubmed|11489127]
  • ''[protein|search|pgk]'': constitutive [Pubmed|11489127]

    • Regulatory mechanism

  • transcription repression by [protein|search|CggR] [Pubmed|11489127]

    • Biological materials

    Mutant

  • GP593 (''pgm''::''cat''), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]
  • GP598 (''pgm''::''erm''), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]
  • GP698 (''pgm''-''eno''::cat), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]

    • Expression vector

  • pGP1425 (expression of ''pgm'' in ''B. subtilis'', in [SW|pBQ200]), available in [SW|Jörg Stülke]'s lab
  • pGP1500 (expression of ''pgm'' and ''eno'' in ''B. subtilis'', in [SW|pBQ200]), available in [SW|Jörg Stülke]'s lab
  • pGP1101 (N-terminal His-tag, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
  • pGP396 (Pgm-S62A, N-terminal His-tag, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
  • pGP92 (N-terminal Strep-tag, for [SW|SPINE], expression in B. subtilis, in [SW|pGP380]), available in [SW|Jörg Stülke]'s lab

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • Labs working on this gene/protein

  • [SW|Jörg Stülke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
  • [Jedrzejas|search|Mark J. Jedrzejas], Research Center Oakland, CA, USA [http://www.chori.org/Principal_Investigators/Jedrzejas_Mark_J/jedrzejas_research.html Homepage]

    • References

  • 11514674,17085493,10764795,8215434,10747010,11712498,9830105,10388626,8636019,11827481,16479537,12850135,17726680,17505547,8021172,11489127,10388626,10747010,10764795,11712498,12729763,17085493,17218307,33963,23420519