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pgm [2019-08-09 12:26:19]
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pgm [2019-08-09 12:26:19]

phosphoglycerate mutase, glycolytic / gluconeogenic enzyme
locus
BSU_33910
pI
5.21
mw
56.14 kDa
protein length
511 aa Sequence Blast
gene length
1536 bp Sequence Blast
function
enzyme in glycolysis / gluconeogenesis
product
2,3-bisphosphoglycerate-independent phosphoglycerate mutase
essential
Yes
ec
5.4.2.1
synonyms
gpmI
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.1|Glycolysis]
  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.2|Gluconeogenesis]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

    • Gene

    Coordinates
    3,477,877 → 3,479,412

    Phenotypes of a mutant

  • Essential [Pubmed|12682299]

    • The protein

    Catalyzed reaction/ biological activity

  • 2-phospho-D-glycerate = 3-phospho-D-glycerate (according to Swiss-Prot)

    • Protein family

  • BPG-independent phosphoglycerate mutase family (single member, according to UniProt)

    • Kinetic information

  • Reversible Michaelis-Menten [Pubmed|33963]

    • Modification

  • phosphorylation on Ser-62 [Pubmed|17218307], [Pubmed|17726680]

    • [SW|Cofactors]

  • Mn2+

    • Effectors of protein activity

  • Inhibited by diverse divalent heavy-metal ions, EDTA and 2,3-butanedione [Pubmed|33963]
  • 2,3-Diphosphoglycerate has NO role on this enzyme regulation [Pubmed|33963]

    • Structure

  • [PDB|1EJJ] (Geobacillus stearothermophilus, complex with 3-phosphoglycerate), [PDB|1EQJ] (Geobacillus stearothermophilus, complex with 2-phosphoglycerate), ''Geobacillus stearothermophilus'', complex with 2-phosphoglycerate [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=16359 NCBI], ''Geobacillus stearothermophilus'', complex with 3-phosphoglycerate [http://www.ncbi.nlm.nih.gov/Structure/mmdb/mmdbsrv.cgi?Dopt=s&uid=15578 NCBI]

    • [SW|Localization]

  • Cytoplasm (Homogeneous) [Pubmed|16479537]

    • Additional information

  • extensive information on the structure and enzymatic properties of Pgm can be found at [http://www.proteopedia.org/wiki/index.php/Phosphoglycerate_Mutase Proteopedia]

    • Expression and Regulation

    Operons

    genes
    [gene|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR]-[gene|EB6512177418B1601C6641FB2DEE99C2CD10E671|gapA]-[gene|ECF0F2E906BF94F509817752827CA189AFBE53FE|pgk]-[gene|0B5E910DC94463E34ABD393E3A8F20191E4A38B2|tpi]-[gene|C86FB523451AC172A20801829B717E5A5F914211|pgm]-[gene|67651D5D3122A89F57F9E7D49074C82A8990A277|eno]
    description
    [Pubmed|11489127]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|11489127], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|CggR]: repression, [Pubmed|11489127], in [regulon|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|CggR regulon]

    • regulation

  • expression induced by glycolytic intermediates ([protein|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|CggR]) [protein|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|CggR] [Pubmed|11489127]
  • the mRNA is processed between [gene|ADA6F2EDC7B18FDFFA47CC8C55BCDDE1B6821160|cggR] and [gene|EB6512177418B1601C6641FB2DEE99C2CD10E671|gapA] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex [Pubmed|29794222]
    • view in new tab

    genes
    [gene|ECF0F2E906BF94F509817752827CA189AFBE53FE|pgk]-[gene|0B5E910DC94463E34ABD393E3A8F20191E4A38B2|tpi]-[gene|C86FB523451AC172A20801829B717E5A5F914211|pgm]-[gene|67651D5D3122A89F57F9E7D49074C82A8990A277|eno]
    description
    [Pubmed|11489127]
    view in new tab

    Biological materials

    Mutant

  • GP593 (Δ''pgm''::''cat''), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]
  • GP598 (Δ''pgm''::''erm''), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]
  • GP698 (Δ''pgm''-''eno''::cat), available in [SW|Jörg Stülke]'s lab, [Pubmed|23420519]

    • Expression vectors

  • pGP1425 (expression of ''pgm'' in ''B. subtilis'', in [SW|pBQ200]), available in [SW|Jörg Stülke]'s lab
  • pGP1500 (expression of ''pgm'' and ''eno'' in ''B. subtilis'', in [SW|pBQ200]), available in [SW|Jörg Stülke]'s lab
  • pGP1101 (N-terminal His-tag, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
  • pGP396 (Pgm-S62A, N-terminal His-tag, in [SW|pWH844]), available in [SW|Jörg Stülke]'s lab
  • pGP92 (N-terminal Strep-tag, for [SW|SPINE], expression in B. subtilis, in [SW|pGP380]), available in [SW|Jörg Stülke]'s lab, [pubmed|19193632]

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab, [pubmed|19193632]

    • labs

  • [SW|Jörg Stülke], University of Göttingen, Germany [http://wwwuser.gwdg.de/~genmibio/stuelke.html Homepage]
  • [Jedrzejas|search|Mark J. Jedrzejas], Research Center Oakland, CA, USA [http://www.chori.org/Principal_Investigators/Jedrzejas_Mark_J/jedrzejas_research.html Homepage]

    • References

  • 11514674,17085493,10764795,8215434,10747010,11712498,9830105,10388626,8636019,11827481,16479537,12850135,17726680,17505547,8021172,11489127,10388626,10747010,10764795,11712498,12729763,17085493,17218307,33963,23420519