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cshB [2017-05-24 09:46:14]
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cshB [2017-05-24 09:46:14]

DEAD-box RNA helicase, important for adaptation to low temperatures
locus
BSU25140
pI
9.89
mw
49.86 kDa
protein length
438 aa Sequence Blast
gene length
1314 bp Sequence Blast
function
RNA helicase
product
DEAD-box RNA helicase
essential
no
synonyms
yqfR
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.3|DEAD-box RNA helicases]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.6|Ribosome assembly]

    • Gene

    Coordinates
    2,594,184 → 2,595,500

    Phenotypes of a mutant

  • poor growth at low temperatures (16 to 20°C) [Pubmed|23175651]

    • The protein

    Catalyzed reaction/ biological activity

  • RNA helicase
  • required for [SW|ribosome] assembly [Pubmed|23175651]

    • Protein family

  • [SW|DEAD-box RNA helicases|DEAD-box RNA helicase]

    • Paralogous protein(s)

  • [protein|E169F9711635457EA65FA71CCE9D7FF29DD8DF02|CshA], [protein|EACD0C84AA3CE41EF05EAA3D25AA1153293EB562|DeaD], [protein|0362D937D985846AEE4D0DEA57B62F915E8D35E5|YfmL]

    • [SW|Localization]

  • cytoplasma, colocalizes with the ribosomes [Pubmed|16352840]

    • Expression and Regulation

    Operons

    genes
    [gene|C83BD80DE0FD187D07B914F64696438ED5130107|cshB]-[gene|3C8BA04BA2BD0FEA28E99DA39BEB710CE3F5922D|nfo]
    description
    [pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-C489 (yqfR::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2487 NBRP B. subtilis, Japan]
  • GP1051 (cat), available in [SW|Jörg Stülke]'s lab

    • Expression vector

  • for expression/ purification from ''B. subtilis'' with C-terminal Strep-tag, for [SW|SPINE], expression from the native chromomsomal site: GP1064 (spc), available in [SW|Jörg Stülke]'s lab

    • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • FLAG-tag construct

  • GP1011 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

    • Labs working on this gene/protein

  • [SW|Mohamed Marahiel], Marburg University, Germany [http://www.uni-marburg.de/fb15/fachgebiete/bio/marahiel?language_sync=1 homepage]

    • References

  • 16352840,12399512,20572937,23175651