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cshB [2019-08-07 12:37:08]
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cshB [2019-08-07 12:37:08]

DEAD-box RNA helicase, important for adaptation to low temperatures
Locus
BSU_25140
Isoelectric point
9.89
Molecular weight
49.86 kDa
Protein length
438 aa Sequence Blast
Gene length
1317 bp Sequence Blast
Function
RNA helicase
Product
DEAD-box RNA helicase
Essential
no
Synonyms
yqfR
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → RNA synthesis and degradation → DEAD-box RNA helicases
  • Information processing → Protein synthesis, modification and degradation → Translation → Ribosome assembly

    • Gene

    Coordinates
    2,594,184 → 2,595,500

    Phenotypes of a mutant

  • poor growth at low temperatures (16 to 20°C) PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • RNA helicase
  • required for ribosome assembly PubMed

    • Protein family

  • helicase family (according to UniProt)
  • DEAD-box RNA helicases (according to UniProt)

    • Paralogous protein(s)

  • CshA, DeaD, YfmL

    • Structure

  • 5IVL (CshA, 80% coverage, 40% identity) PubMed

    • Localization

  • cytoplasma, colocalizes with the ribosomes PubMed

    • Expression and Regulation

    Operons

    Genes
    cshB-nfo
    Description
    PubMed
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    Biological materials

    Mutant

  • MGNA-C489 (cshB::erm), available at the NBRP B. subtilis, Japan
  • GP1051 (ΔcshB::cat), available in Jörg Stülke's lab
  • BKE25140 (ΔcshB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTTCCCAGCTCCTTAA, downstream forward: _UP4_AAGAAAAGAAAGTAGGGGAA
  • BKK25140 (ΔcshB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTTTCCCAGCTCCTTAA, downstream forward: _UP4_AAGAAAAGAAAGTAGGGGAA

    • Expression vectors

  • for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, expression from the native chromomsomal site: GP1064 (spc), available in Jörg Stülke's lab

    • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab, PubMed

    • FLAG-tag construct

  • GP1011 (spc, based on pGP1331), available in Jörg Stülke's lab

    • Labs working on this gene/protein

  • Mohamed Marahiel, Marburg University, Germany homepage

    • References

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