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cshB [Tue Dec 13 2016 11:16:35 GMT+0100 (CET)]
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cshB [Tue Dec 13 2016 11:16:35 GMT+0100 (CET)]

DEAD-box RNA helicase, important for adaptation to low temperatures
locus
BSU25140
pI
9.89
mw
49.00 kDa
function
RNA helicase
product
DEAD-box RNA helicase
essential
no
synonyms
yqfR
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.3|DEAD-box RNA helicases]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.6|Ribosome assembly]

    • [SW|Categories] containing this gene/protein

  • [SW|DEAD-box RNA helicases], [SW|translation]

    • Gene

    Coordinates on the chromosome (coding sequence)
    2,594,184 -> 2,595,500

    Phenotypes of a mutant

  • poor growth at low temperatures (16 to 20°C) [Pubmed|23175651]

    • The protein

    Catalyzed reaction/ biological activity

  • RNA helicase
  • required for [SW|ribosome] assembly [Pubmed|23175651]

    • Protein family

  • [DEAD-box RNA helicases|search|DEAD-box RNA helicase]

    • Paralogous protein(s)

  • [protein|search|CshA], [protein|search|DeaD], [protein|search|YfmL]

    • [SW|Localization]

  • cytoplasma, colocalizes with the ribosomes [Pubmed|16352840]

    • [SW|Interactions]

  • [protein|search|CshB]-[protein|search|CspB] [Pubmed|16352840]
  • [protein|search|CshB]-[protein|search|DeaD] [Pubmed|20572937]

    • Expression and Regulation

    Regulation

  • well expressed in complex medium, poorly expressed in minimal medium [Pubmed|20572937]
  • expression declines in stationary phase [Pubmed|23175651,20572937]

    • Biological materials

    Mutant

  • GP1051 (cat), available in [SW|Jörg Stülke]'s lab

    • Expression vector

  • for expression/ purification from ''B. subtilis'' with C-terminal Strep-tag, for [SW|SPINE], expression from the native chromomsomal site: GP1064 (spc), available in [SW|Jörg Stülke]'s lab

    • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • FLAG-tag construct

  • GP1011 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

    • Labs working on this gene/protein

  • [SW|Mohamed Marahiel], Marburg University, Germany [http://www.uni-marburg.de/fb15/fachgebiete/bio/marahiel?language_sync=1 homepage]

    • References

  • 16352840,12399512,20572937,23175651