transcription activator of gabT-gabD, repressor of gabR (MocR/ GabR family)
function
regulation of gamma-amino butyric acid utilization
product
transcription regulator
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.10|Utilization of gamma-amino butyric acid][category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other][SW|Categories] containing this gene/protein
[SW|utilization of amino acids], [SW|transcription factors and their control]This gene is a member of the following [SW|regulons]
[SW|GabR regulon]Gene
Coordinates on the chromosome (coding sequence)
440,025 -> 441,464
The protein
Catalyzed reaction/ biological activity
transcription activation of the ''[protein|search|gabT]-[protein|search|gabD]'' operon in the presence of GABA and PLP, transcription repression of ''[protein|search|gabR]'' [Pubmed|15223311]Protein family
[[MocR/ GabR family]] [Pubmed|22020104]Paralogous protein(s)
none, but there are 7 members of the [[MocR/ GabR family]] in ''B. subtilis[SW|Domains]
N-terminal DNA-binding helix-turn-helix motif (corresponding to domains of the [SW|GntR family]) [Pubmed|24127574]C-terminal domain is homologous to PLP-binding large domain of aminotransferases [Pubmed|24127574][SW|Cofactors]
gamma-aminobutyric acid, PLP [Pubmed|24127574]Effectors of protein activity
activation of ''[protein|search|gabT]-[protein|search|gabD]'' expression is triggered by gamma-amino butyrate and pyridoxalphosphate [Pubmed|15223311]Structure
[PDB|4MGR] (the apo-protein) [Pubmed|24127574][PDB|4N0B] (the complex with PLP) [Pubmed|24127574][SW|Interactions]
forms head-to-tail domain-swapped dimers [Pubmed|24127574]Expression and Regulation
Operon
''[protein|search|gabR]'' (according to [http://dbtbs.hgc.jp/COG/prom/gabR.html DBTBS])[SW|Sigma factor]
[protein|search|SigA] [Pubmed|12123465]Regulatory mechanism
GabR: negative autoregulation [Pubmed|12123465]Biological materials
Mutant
MGNA-C014 (ycnF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2012 NBRP B. subtilis, Japan]Labs working on this gene/protein
[SW|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]References
11756427,15223311,12123465,24127574,22020104,25388514,25911692,27640111 
