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yvrC [2020-06-18 09:50:51]
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yvrC [2020-06-18 09:50:51]

[SW|ABC transporter] (binding protein) for cobalamin
locus
BSU_33180
pI
4.93
mw
34.08 kDa
protein length
314 aa Sequence Blast
gene length
945 bp Sequence Blast
function
uptake of cobalamin
product
[SW|ABC transporter] (binding protein)
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.7|Uptake of cofactors]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.13|Biosynthesis/ acquisition of cobalamine (B12))]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    3,403,493 → 3,404,437

    The protein

    Protein family

  • [SW|Bacterial solute-binding protein 8 family] (according to UniProt)

    • [SW|Domains]

  • [SW|Fe/B12 periplasmic-binding domain] (aa 59-311) (according to UniProt)

    • Structure

  • [PDB|4M7O] (from Staphylococcus epidermidis, 45% identity)

    • [SW|Localization]

  • cell membrane (according to UniProt)

    • Expression and Regulation

    Operons

    genes
    [gene|C76265F521D9A153F28AC6BACD935CC20DEA5D9C|yvrC]-[gene|1DC4A3D7E93677B0EAD15F43C80E50FD98527EBC|yvrB]-[gene|128EBAE9C14C7BB75173DF912853A6FCE86ABCE4|yvrA]-[gene|468D9141EAC78556C9F6C564875F70EC43E60C1C|yvqK]

    regulatory mechanism

  • [regulon|B12 riboswitch|B12 riboswitch]: termination, upon binding of B12 [Pubmed|14704351], in [regulon|B12 riboswitch|B12 riboswitch]

    • regulation

  • expression is decreased in the presence of cobalamin (vitamin B12) ([SW|B12 riboswitch]) [Pubmed|14704351]
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    Biological materials

    Mutant

  • MGNA-B045 (yvrC::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1044 NBRP B. subtilis, Japan]
  • BKE33180 (Δ[gene|C76265F521D9A153F28AC6BACD935CC20DEA5D9C|yvrC]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE33180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATCTTTTCCTCCTAAA, downstream forward: _UP4_CGTCTGATCGAAGGGGTCGA
  • BKK33180 (Δ[gene|C76265F521D9A153F28AC6BACD935CC20DEA5D9C|yvrC]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK33180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATCTTTTCCTCCTAAA, downstream forward: _UP4_CGTCTGATCGAAGGGGTCGA

    • References

  • 10092453,22383849,14704351