yqiD [2019-08-07 11:13:22]

geranyltransferase

locus: BSU_24280

pI: 5.02

mw: 29.59 kDa

protein length: 296 aa

gene length: 891 bp

function: biosynthesis of peptidoglycan and teichoic acid

product: geranyltransferase

essential: no

ec: 2.5.1.10

synonyms: ispA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.3|Biosynthesis of isoprenoids]

Gene

Coordinates: 2,525,789 → 2,526,679

Phenotypes of a mutant

• The mutation of ''yqiD'' predisposes cells to grow without a wall [Pubmed|19212404]

The protein

Catalyzed reaction/ biological activity

• dimethylallyl pyrophosphate 2 isopentanyl pyrophosphate → farnasyl pyrophosphate

Protein family

• FPP/GGPP synthase family (with [protein|3CAEA54E80A0B56EE120B881C8CDBE055AF6BDC1|HepT], according to UniProt)

Paralogous protein(s)

• [protein|3CAEA54E80A0B56EE120B881C8CDBE055AF6BDC1|HepT]

Modification

• None

[SW|Cofactors]

• Magnesium

Structure

• [PDB|1RQI]: IspA from ''E. coli''. Co-crystal with substrate isopentyl pyrophosphate and substrate analogue dimethyallyl S-thiolodiphosphate [Pubmed|14672944]

Biological materials

Mutant

• BKE24280 (Δ[gene|C75A4465688BCE9902F9CDC6DE069E36667377E1|yqiD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE24280 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CCGGTCCGCCAGAAAGCTCG, downstream forward: _UP4_TAAAACGATCCTAATTTGAA
• BKK24280 (Δ[gene|C75A4465688BCE9902F9CDC6DE069E36667377E1|yqiD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK24280 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CCGGTCCGCCAGAAAGCTCG, downstream forward: _UP4_TAAAACGATCCTAATTTGAA

References

• 14672944,17114254,19212404,17458547,26051891