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wapI
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wapI

immunity protein, protects the cell against the toxic activity of WapA
Locus
BSU_39220
Isoelectric point
4.42
Molecular weight
16.29 kDa
Protein length
142 aa Sequence Blast
Gene length
429 bp Sequence Blast
Function
intercellular competition
Product
immunity protein
Essential
yes
Synonyms
yxxG

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
4,023,054 → 4,023,482

Phenotypes of a mutant

  • essential PubMed
  • severe growth defect PubMed
  • The protein

    Catalyzed reaction/ biological activity

  • WapI inhibits the toxic activity of WapA PubMed
  • Modification

  • phosphorylation on (Tyr-102 OR Ser-105) PubMed
  • Expression and Regulation

    Operons

    Genes
    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • Regulatory mechanism

  • DegU: repression, DegU-P, PubMed, in DegU regulon
  • YvrHb: activation, PubMed, in YvrHb regulon
  • WalR: repression, PubMed, in WalR regulon
  • Regulation

  • repressed at high salt concentration (DegU-P) PubMed
  • Additional information

  • PhoP∼P binds to the promoter region, but regulation has not been explored PubMed
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    Biological materials

    Mutant

  • MGNA-B789 (yxxG::erm), available at the NBRP B. subtilis, Japan
  • BKE39220 (ΔwapI::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTTCCTCCTCATTTAA, downstream forward: _UP4_TAATTATAAATAATTTCTGC
  • BKK39220 (ΔwapI::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTTCCTCCTCATTTAA, downstream forward: _UP4_TAATTATAAATAATTTCTGC
  • References

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