ATP-dependent DNA helicase, facilitates unwinding of ICEBs1 DNA for horizontal transfer
function
plasmid rolling-circle replication, conjugative transfer of ICEBs1
product
ATP-dependent DNA helicase
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination][category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins][category|SW 5|Prophages and mobile genetic elements] → [category|SW 5.2|Mobile genetic elements] → [category|SW 5.2.2|Additional genes][category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes][SW|Categories] containing this gene/protein
[SW|DNA repair/ recombination], [SW|mobile genetic elements], [SW|essential genes]This gene is a member of the following [SW|regulons]
[SW|LexA regulon]Gene
Phenotypes of a mutant
essential [Pubmed|12682299]The protein
Catalyzed reaction/ biological activity
PcrA anchors itself to the template duplex using the 2B subdomain and reels in the lagging strand, extruding a single-stranded loop [Pubmed|20723756]dismantles [protein|search|RecA] filaments [Pubmed|20723756]Protein family
uvrD-like helicase C-terminal domain (according to Swiss-Prot)Paralogous protein(s)
[protein|search|YjcD][SW|Domains]
two RecA-like domains [Pubmed|24147116]intrinxically disordered C-terminal domain (for interaction with [SW|RNA polymerase]) [Pubmed|24147116]Effectors of protein activity
unwinding of the double-stranded ICEBs1 DNA is stimulated by [protein|search|HelP] [Pubmed|23326247]helicase activity is stimulated by interaction with [SW|RNA polymerase] [Pubmed|24147116]Structure
[PDB|2PJR] (product complex, complex with sulphate ion, ''Geobacillus stearothermophilus'')[PDB|1PJR] (substrate complex, complex with DNA, ''Geobacillus stearothermophilus'')[SW|Interactions]
the C-terminal domain of [protein|search|PcrA] interacts with the [SW|RNA polymerase] [Pubmed|24147116,21710567][protein|search|YxaL]-[protein|search|PcrA] [Pubmed|12073041][protein|search|YerB]-[protein|search|PcrA] [Pubmed|12073041][protein|search|YwhK]-[protein|search|PcrA] [Pubmed|12073041][protein|search|UvrB]-[protein|search|PcrA] [Pubmed|24147116][protein|search|YwqA]-[protein|search|PcrA] [Pubmed|24147116]Expression and Regulation
Operon
''[protein|search|pcrB]-[protein|search|pcrA]-[protein|search|ligA]-[protein|search|yerH]'' [Pubmed|9701819]Regulation
induced by DNA damage ([protein|search|LexA]) [Pubmed|16267290]Regulatory mechanism
[protein|search|LexA]: transcription repression [Pubmed|16267290]Biological materials
two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Fabian Commichau]'s labReferences
Reviews
Original Publications
12073041,9701819,16267290,19943900,20723756,18276648,17449621,10899133,10199404,23326247,24147116,21710567,26070154