SubtiBank SubtiBank
pcrA [Mon Aug 31 2015 13:52:57 GMT+0200 (CEST)]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

pcrA [Mon Aug 31 2015 13:52:57 GMT+0200 (CEST)]

ATP-dependent DNA helicase, facilitates unwinding of ICEBs1 DNA for horizontal transfer
locus
BSU06610
pI
5.77
mw
83.00 kDa
function
plasmid rolling-circle replication, conjugative transfer of ICEBs1
product
ATP-dependent DNA helicase
essential
yes
synonyms
yerF

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
  • [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]
  • [category|SW 5|Prophages and mobile genetic elements] → [category|SW 5.2|Mobile genetic elements] → [category|SW 5.2.2|Additional genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • syns
    yerF

    [SW|Categories] containing this gene/protein

  • [SW|DNA repair/ recombination], [SW|mobile genetic elements], [SW|essential genes]
  • This gene is a member of the following [SW|regulons]

  • [SW|LexA regulon]
  • Gene

    Phenotypes of a mutant

  • essential [Pubmed|12682299]
  • The protein

    Catalyzed reaction/ biological activity

  • PcrA anchors itself to the template duplex using the 2B subdomain and reels in the lagging strand, extruding a single-stranded loop [Pubmed|20723756]
  • dismantles [protein|search|RecA] filaments [Pubmed|20723756]
  • Protein family

  • uvrD-like helicase C-terminal domain (according to Swiss-Prot)
  • Paralogous protein(s)

  • [protein|search|YjcD]
  • [SW|Domains]

  • two RecA-like domains [Pubmed|24147116]
  • intrinxically disordered C-terminal domain (for interaction with [SW|RNA polymerase]) [Pubmed|24147116]
  • Effectors of protein activity

  • unwinding of the double-stranded ICEBs1 DNA is stimulated by [protein|search|HelP] [Pubmed|23326247]
  • helicase activity is stimulated by interaction with [SW|RNA polymerase] [Pubmed|24147116]
  • Structure

  • [PDB|2PJR] (product complex, complex with sulphate ion, ''Geobacillus stearothermophilus'')
  • [PDB|1PJR] (substrate complex, complex with DNA, ''Geobacillus stearothermophilus'')
  • [SW|Interactions]

  • the C-terminal domain of [protein|search|PcrA] interacts with the [SW|RNA polymerase] [Pubmed|24147116,21710567]
  • [protein|search|YxaL]-[protein|search|PcrA] [Pubmed|12073041]
  • [protein|search|YerB]-[protein|search|PcrA] [Pubmed|12073041]
  • [protein|search|YwhK]-[protein|search|PcrA] [Pubmed|12073041]
  • [protein|search|UvrB]-[protein|search|PcrA] [Pubmed|24147116]
  • [protein|search|YwqA]-[protein|search|PcrA] [Pubmed|24147116]
  • Expression and Regulation

    Operon

  • ''[protein|search|pcrB]-[protein|search|pcrA]-[protein|search|ligA]-[protein|search|yerH]'' [Pubmed|9701819]
  • Regulation

  • induced by DNA damage ([protein|search|LexA]) [Pubmed|16267290]
  • Regulatory mechanism

  • [protein|search|LexA]: transcription repression [Pubmed|16267290]
  • Biological materials

    two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Fabian Commichau]'s lab
  • References

    Reviews

  • 19943906
  • Original Publications

  • 12073041,9701819,16267290,19943900,20723756,18276648,17449621,10899133,10199404,23326247,24147116,21710567,26070154