pcrA

ATP-dependent DNA helicase, coupling of DNA replication, transcription, recombination and segregation, facilitates unwinding of ICEBs1 DNA for horizontal transfer

locus: BSU_06610

pI: 5.77

mw: 83.37 kDa

protein length: 739 aa

gene length: 2220 bp

function: coupling of DNA replication, transcription, recombination and segregation

product: ATP-dependent DNA helicase

essential: yes

synonyms: yerF

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]
• [category|SW 5|Prophages and mobile genetic elements] → [category|SW 5.2|Mobile genetic elements] → [category|SW 5.2.2|Additional genes]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 719,370 → 721,589

Phenotypes of a mutant

• essential [Pubmed|12682299]
• inactivation can be suppressed by inactivation of either [gene|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA], [gene|BA755FA1CB1E0C006E9A23489A7C8997141AA498|recJ], [gene|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO], or [gene|AD1F39823ABCE2A222259B879513752942C59590|mfd] [pubmed|32793628]

The protein

Catalyzed reaction/ biological activity

• coupling of DNA replication, transcription, recombination and segregation [pubmed|32793628]
• PcrA anchors itself to the template duplex using the 2B subdomain and reels in the lagging strand, extruding a single-stranded loop [Pubmed|20723756]
• dismantles [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|RecA] filaments [Pubmed|20723756]
• ATP + H₂O --> ADP + H⁺ + phosphate (according to UniProt)

Protein family

• [SW|helicase family] (according to UniProt)

Paralogous protein(s)

• [protein|B0789A727D9E3578FF1CB6162F365F45FADB0B3E|YjcD]

[SW|Domains]

• two RecA-like domains [Pubmed|24147116]
• intrinxically disordered C-terminal domain (for interaction with [SW|RNA polymerase]) [Pubmed|24147116]
• [SW|UvrD-like helicase ATP-binding domain](aa 10-289) (according to UniProt)
• [SW|UvrD-like helicase C-terminal] (aa 290-570) (according to UniProt)

Effectors of protein activity

• unwinding of the double-stranded ICEBs1 DNA is stimulated by [protein|search|HelP] [Pubmed|23326247]
• helicase activity is stimulated by interaction with [SW|RNA polymerase] [Pubmed|24147116]

Structure

• [PDB|2PJR] (product complex, complex with sulphate ion, ''Geobacillus stearothermophilus'')
• [PDB|1PJR] (substrate complex, complex with DNA, ''Geobacillus stearothermophilus'')
• [PDB|5DMA], C-terminal [SW|RNA polymerase] interaction domain [pubmed|28160601]

Expression and Regulation

Operons

• genes: [gene|0BA7EC658F5C2397A90B68AC234D04966966561C|pcrB]-[gene|C73E52D214E24F21696973B19B0A44CE785D6FBA|pcrA]-[gene|03EFF93C1B0CE075AE068ECC4EDE9BE5D03C857E|ligA]-[gene|ADAE6B5C301959D90592A6F26DFE1690BF6F5552|yerH]
  description: [Pubmed|9701819]

  regulatory mechanism

  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA]: repression, [Pubmed|16267290], in [regulon|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|LexA regulon]

  regulation

  • induced by DNA damage ([protein|search|LexA]) [Pubmed|16267290]

Biological materials

two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Fabian Commichau]'s lab

References

Reviews

• 19943906,29856930

Original Publications

• 12073041,9701819,16267290,19943900,20723756,18276648,17449621,10899133,10199404,23326247,24147116,21710567,26070154,28160601,32793628