yknV

similar to [SW|ABC transporter] (ATP-binding protein)

locus: BSU_14330

pI: 6.69

mw: 67.75 kDa

protein length: 604 aa

gene length: 1815 bp

function: unknown

product: [SW|ABC transporter] (ATP-binding protein)

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.2|Exporters] → [category|SW 1.2.1.2.6|Exporters of unknown function]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 1,501,658 → 1,503,472

The protein

Protein family

• [SW|ABC transporter superfamily] (according to UniProt)

Paralogous protein(s)

• [protein|D5A3C316BD4567A0155F70330E32ACDE18F063FE|YwjA], [protein|E85814EDF232D21A42380D559EAD2CDFB41F19DB|YfiC], [protein|ED1F82463BAA28B67184BFAB5CB23CF26A62999D|BmrA], [protein|70D7098BEBD5E53B4B9B8C3394FAAF972C65EE22|YknU], [protein|67C0C9EA17EF953554D3D57CD0D285246F50C1AD|BmrC], [protein|288CA73D93B1E5106E1C6CE5E7E2E8BE2A6BD3F3|YfiB], [protein|0EB4D3E1B9AF39ECCBD79EB559FAE5BB0EE4C156|YgaD]

[SW|Domains]

• has both a membrane-spanning and an ATP-binding domain [Pubmed|10092453]
• [SW|ABC transmembrane type-1 domain] (aa 45-329) (according to UniProt)
• [SW|ABC transporter domain] (aa 363-597) (according to UniProt)

Structure

• [PDB|5MKK] (TmrAB complex from Thermus thermophilus, 35% identity) [pubmed|28069938]

[SW|Localization]

• cell membrane [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|70D7098BEBD5E53B4B9B8C3394FAAF972C65EE22|yknU]-[gene|C6B0ACB9D9703DD8141FD7D9D62DB22FB48F458B|yknV]-[gene|916419C4ED113778488EBC558A35810498028B02|yknW]-[gene|FD270195901B89DAAAD134DB9DE729670A9BDECF|yknX]-[gene|FE3A01BEA974C5E32C68DC600C800E5EF101B34F|yknY]-[gene|9680326BAA94A503E2A5C6F0A2479AE0B0467B4C|yknZ]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B347 (yknV::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1346 NBRP B. subtilis, Japan]
• BKE14330 (Δ[gene|C6B0ACB9D9703DD8141FD7D9D62DB22FB48F458B|yknV]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE14330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGCTTGTTTCATTTGGCCCC, downstream forward: _UP4_TAATATGAAAAGCCTTCAAT
• BKK14330 (Δ[gene|C6B0ACB9D9703DD8141FD7D9D62DB22FB48F458B|yknV]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK14330 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CGCTTGTTTCATTTGGCCCC, downstream forward: _UP4_TAATATGAAAAGCCTTCAAT

References

• 10092453,28069938