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malA [2019-07-25 09:41:40]
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malA [2019-07-25 09:41:40]

6-phospho-alpha-glucosidase
locus
BSU_08180
pI
4.74
mw
50.35 kDa
protein length
449 aa Sequence Blast
gene length
1350 bp Sequence Blast
function
maltose utilization
product
6-phospho-alpha-glucosidase
essential
no
ec
3.2.1.122
synonyms
glvG,glvA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.22|Utilization of maltose]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    890,022 → 891,371

    The protein

    Catalyzed reaction/ biological activity

  • Maltose 6'-phosphate + H2O = D-glucose + D-glucose 6-phosphate (according to Swiss-Prot)

    • Protein family

  • [SW|Glycosyl hydrolase 4 family] (according to UniProt)

    • Structure

  • [PDB|1U8X]

    • [SW|Localization]

  • membrane associated [Pubmed|18763711]

    • Expression and Regulation

    Operons

    genes
    [gene|C650160C9E5B5F8587CA9E80A57BCCE97D701037|malA]-[gene|AD72489DA260F07B97A213F1F0FBA7CED9471817|glvR]-[gene|E6C7733A12AF9A5322EED444E4A4FF1605A8B20F|malP]
    description
    [Pubmed|11489864]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|11489864], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|AD72489DA260F07B97A213F1F0FBA7CED9471817|GlvR]: activation, [Pubmed|11489864], in [regulon|AD72489DA260F07B97A213F1F0FBA7CED9471817|GlvR regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|11489864], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

    • regulation

  • induction by maltose ([protein|search|GlvR]) [Pubmed|11489864]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-C289 (glvA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2287 NBRP B. subtilis, Japan]
  • BKE08180 (Δ[gene|C650160C9E5B5F8587CA9E80A57BCCE97D701037|malA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGACGACCTCCTTGAT, downstream forward: _UP4_TAAATGAAATTCCCCCATTT
  • BKK08180 (Δ[gene|C650160C9E5B5F8587CA9E80A57BCCE97D701037|malA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGACGACCTCCTTGAT, downstream forward: _UP4_TAAATGAAATTCCCCCATTT

    • References

  • 22900538,16707683,10627040,18763711,9765262,11489864,17676871,15341727,20618987