mstX

membrane-integrating protein for membrane protein expression, MISTIC

Locus: BSU_31321

Isoelectric point: 4.00

Molecular weight: 13.00 kDa

Protein length: 110 aa

Gene length: 333 bp

Function: unknown

Product: MISTIC

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Protein secretion
• Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation
• Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation → Regulation
• Lifestyles → Sporulation → Sporulation proteins → Newly identified sporulation proteins (based on transcription profiling)
• Groups of genes → Membrane proteins

Gene

Coordinates: 3,218,525 → 3,218,857

Phenotypes of a mutant

• reduced biofilm formation (can be suppressed by inactivation of sinR) PubMed
• overexpression result in increased biofilm formation even in a domesticated strain background PubMed

The protein

Structure

• 1YGM PubMed

Localization

• integral membrane protein that folds autonomously into the membrane, bypassing the cellular translocon machinery PubMed

Expression and Regulation

Operons

• Genes: mstX-yugO
  Description: PubMed

  Regulatory mechanism

  • SinR: repression, PubMed, in SinR regulon

  Regulation

  • repressed by SinR PubMed

  Additional information

  • potassium exporter activity of YugO is activated by glutamate limitation via the C-terminal domain PubMed

Biological materials

Mutant

• BKE31321 (ΔmstX::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATAAAACCGGATTTCTC, downstream forward: _UP4_GTATCTGAAGAAGGAGAAAA
• BKK31321 (ΔmstX::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATAAAACCGGATTTCTC, downstream forward: _UP4_GTATCTGAAGAAGGAGAAAA

References

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