fin

RNA polymerase-binding protein, feedback inhibitor of SigF activity

Locus: BSU_00540

Isoelectric point: 5.19

Molecular weight: 8.71 kDa

Protein length: 76 aa

Gene length: 231 bp

Function: control of sporulation

Product: feedback inhibitor of SigF activity

Essential: no

Synonyms: subA,yabK

Genomic Context

Loading

Categories containing this gene/protein

• Information processing → Genetics → DNA repair/ recombination
• Information processing → Genetics → DNA repair/ recombination → Other proteins
• Information processing → Regulation of gene expression → Sigma factors and their control → Control of sigma factors
• Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other

Gene

Coordinates: 60,130 → 60,360

Phenotypes of a mutant

• sporulation defect (50-fold reduction of viable spore count) and arrest after engulfment PubMed

The protein

Catalyzed reaction/ biological activity

• binding of Fin to the ß' subunit of RNA polymerase prevents binding of SigF, and thus SigF-dependent transcription PubMed

Structure

• 5MSL PubMed

Localization

• localizes diffusely within the forespore compartment of sporangia at both early and late stages of sporulation, cytosolic protein PubMed

Expression and Regulation

Operons

• Genes: fin
  Description: PubMed

  Sigma factors

  • SigF: sigma factor, PubMed, in SigF regulon
  • SigG: sigma factor, PubMed, in SigG regulon

  Regulation

  • expressed in two waves in the forespore (after two hours (SigF) and three hours (SigG) after the onset of sporulation) PubMed

• Genes: ctc-spoVC-fin
  Description: PubMed

  Sigma factors

  • SigB: sigma factor, PubMed, in SigB regulon

  Regulation

  • induced under stress conditions (SigB) PubMed

• Genes: fin-mfd-spoVT
  Description: PubMed

Biological materials

Mutant

• MGNA-B913 (yabK::erm), available at the NBRP B. subtilis, Japan
• BKE00540 (Δfin::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATACAAGGGTCCTCCTGAT, downstream forward: _UP4_TAAAAAGCTTTGGTGTAGAC
• BKK00540 (Δfin::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATACAAGGGTCCTCCTGAT, downstream forward: _UP4_TAAAAAGCTTTGGTGTAGAC

References

Reviews

Loading

Original Publications

Loading