fin

[SW|RNA polymerase]-binding protein, feedback inhibitor of [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF] activity

locus: BSU_00540

pI: 5.19

mw: 8.71 kDa

protein length: 76 aa

gene length: 231 bp

function: control of [SW|sporulation]

product: feedback inhibitor of [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF] activity

essential: no

synonyms: subA,yabK

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination]
• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.5|DNA repair/ recombination] → [category|SW 3.1.5.6|Other proteins]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.1|Sigma factors and their control] → [category|SW 3.4.1.2|Control of sigma factors]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.4|Sporulation proteins/ other]

Gene

Coordinates: 60,130 → 60,360

Phenotypes of a mutant

• sporulation defect (50-fold reduction of viable spore count) and arrest after engulfment [Pubmed|21037003]

The protein

Catalyzed reaction/ biological activity

• binding of Fin to the [protein|BC8B37355E6FADBA5F4636C3879E99003B0F3E84|ß'] subunit of [SW|RNA polymerase] prevents binding of [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF], and thus [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]-dependent transcription [pubmed|28598017]

Structure

• [PDB|5MSL] [pubmed|28598017]

[SW|Localization]

• localizes diffusely within the forespore compartment of sporangia at both early and late stages of sporulation, cytosolic protein [Pubmed|21037003]

Expression and Regulation

Operons

• genes: [gene|C595D32D98723E93AF26B5C864FD61AF4739E069|fin]
  description: [pubmed|22383849]

  sigma factors

  • [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]: sigma factor, [Pubmed|21037003], in [regulon|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF regulon]
  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|21037003], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]

  regulation

  • expressed in two waves in the forespore (after two hours ([protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF]) and three hours ([protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]) after the onset of sporulation) [Pubmed|21037003]

• genes: [gene|40624E9FC30D75D172EF098C7FAC77B2573CC129|ctc]-[gene|FE7859E98F26E532AE02CD71B42B845BADBCF06E|spoVC]-[gene|C595D32D98723E93AF26B5C864FD61AF4739E069|fin]
  description: [pubmed|22383849]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [pubmed|15805528], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

  regulation

  • induced under stress conditions ([protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]) [pubmed|15805528]

• genes: [gene|C595D32D98723E93AF26B5C864FD61AF4739E069|fin]-[gene|AD1F39823ABCE2A222259B879513752942C59590|mfd]-[gene|01EF886D3D004EA9B22AC5E75D50FF07D8631E68|spoVT]
  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-B913 (yabK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1912 NBRP B. subtilis, Japan]
• BKE00540 (Δ[gene|C595D32D98723E93AF26B5C864FD61AF4739E069|fin]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE00540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACAAGGGTCCTCCTGAT, downstream forward: _UP4_TAAAAAGCTTTGGTGTAGAC
• BKK00540 (Δ[gene|C595D32D98723E93AF26B5C864FD61AF4739E069|fin]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK00540 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACAAGGGTCCTCCTGAT, downstream forward: _UP4_TAAAAAGCTTTGGTGTAGAC

References

Reviews

• 31350897

Original Publications

• 15317759,11810266,21037003,28598017