reoM [2020-06-03 15:07:16]

regulator of [protein|DA763F7C2192655C590752C2A285ADF902B11BCA|MurAA] degradation

locus: BSU_27400

pI: 4.73

mw: 10.10 kDa

protein length: 88 aa

gene length: 267 bp

function: unknown

product: unknown

essential: no

synonyms: yrzL

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.5|Cell wall/ other]

Gene

Coordinates: 2,797,823 → 2,798,089

The protein

Protein family

• UPF0297 family (single member, according to UniProt)

Structure

• [PDB|6TIF] (from Listeria monocytogenes, 68% identity) [pubmed|32469310]
• [PDB|5US5] (from Enterococcus faecalis, 66% identity) [pubmed|28551334]

Expression and Regulation

Operons

• genes: [gene|C5457FEC46A1AFC6E5B1148B0E5F258B2C3197B2|reoM]-[gene|508FC7117B4CE7AF119B3830AE9DC113CB058039|yrrK]-[gene|A0AA5289F5E92A4B2E18E4F3439F2D412208826E|yrzB]
  description: [pubmed|22383849]

• genes: [gene|8C5C7F9A585AE89D53EAF1DB3A9AB525B9E30B4A|alaS]-[gene|C5457FEC46A1AFC6E5B1148B0E5F258B2C3197B2|reoM]-[gene|508FC7117B4CE7AF119B3830AE9DC113CB058039|yrrK]-[gene|A0AA5289F5E92A4B2E18E4F3439F2D412208826E|yrzB]
  description: [pubmed|22383849]

  regulatory mechanism

  • [regulon|T-box|T-box]: antitermination, in [regulon|T-box|T-box]

  regulation

  • induced by alanine limitation ([regulon|T-box|T-box]) [Pubmed|19258532]

Biological materials

Mutant

• BKE27400 (Δ[gene|C5457FEC46A1AFC6E5B1148B0E5F258B2C3197B2|reoM]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE27400 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCGTTTTGCACCTCTTTC, downstream forward: _UP4_CAGCATAAAGAGGCGTAAAT
• BKK27400 (Δ[gene|C5457FEC46A1AFC6E5B1148B0E5F258B2C3197B2|reoM]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK27400 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACCGTTTTGCACCTCTTTC, downstream forward: _UP4_CAGCATAAAGAGGCGTAAAT

References

Research papers

• 32469310,28551334