mifM

ribosome-nascent chain sensor of membrane protein biogenesis

locus: BSU_23880

pI: 5.83

mw: 11.01 kDa

protein length: 95 aa

gene length: 288 bp

function: control of yidC2 translation

product: sensor of SpoIIIJ activity

essential: no

synonyms: yqzJ

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.12|Translation/ other]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 2,483,586 → 2,483,873

The protein

Catalyzed reaction/ biological activity

• acts as a kind of "leader peptide" that does or does not allow translation through a hairpin structure in the ''[gene|121A9543F5BC1CBE77D85A34099DE09F8AC63E9F|yidC2]'' mRNA [Pubmed|19779460]

[SW|Domains]

• N-terminal transmembrane domain [Pubmed|22864117]
• the C-terminal domain interacts with the ribosome and arrests the own translation elongation [Pubmed|22864117]

Structure

• [PDB|3J9W] ([protein|C50AE06E8C959EB7176504C8E2BAD1A08E47C51A|MifM]-stalled [SW|ribosome] complex) [Pubmed|25903689]
• [http://www.ebi.ac.uk/pdbe/entry/emdb/EMD-6306 EMD-6306] ([protein|C50AE06E8C959EB7176504C8E2BAD1A08E47C51A|MifM]-stalled [SW|ribosome] complex) [Pubmed|25903689]

[SW|Localization]

• membrane [Pubmed|19779460]

Expression and Regulation

Operons

• genes: [gene|C50AE06E8C959EB7176504C8E2BAD1A08E47C51A|mifM]-[gene|121A9543F5BC1CBE77D85A34099DE09F8AC63E9F|yidC2]
  description: [Pubmed|22383849]

  regulatory mechanism

  • [protein|C50AE06E8C959EB7176504C8E2BAD1A08E47C51A|MifM]: attenuation, an mRNA hairpin of the yqjG transcript unfold upon translation arrest of [protein|C50AE06E8C959EB7176504C8E2BAD1A08E47C51A|MifM] at decreased [protein|D40C202E67A5319A91811DB11356F47A56C97DD2|YidC1] levels, in [regulon|C50AE06E8C959EB7176504C8E2BAD1A08E47C51A|MifM regulon]

  regulation

  • repressed by large amounts of [SW|SpoIIIJ], induced by small amounts of [SW|SpoIIIJ] ([protein|search|MifM]) [Pubmed|19779460]

  additional information

  • An [ncRNA|search|antisense RNA] is predicted for '[protein|search|mifM]' [PubMed|20525796]

Biological materials

Mutant

• BKE23880 (Δ[gene|C50AE06E8C959EB7176504C8E2BAD1A08E47C51A|mifM]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACATCCATTCAATAGC, downstream forward: _UP4_TAAACCGCATTTATAAAAAG
• BKK23880 (Δ[gene|C50AE06E8C959EB7176504C8E2BAD1A08E47C51A|mifM]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23880 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACATCCATTCAATAGC, downstream forward: _UP4_TAAACCGCATTTATAAAAAG

References

• 19779460,21383133,20525796,22456704,22383849,22864117,25313395,25903689,29985442