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trmK [2019-04-02 12:07:01]
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trmK [2019-04-02 12:07:01]

tRNA:m1A22 methyl transferase
Locus
BSU25180
Isoelectric point
5.27
Molecular weight
23.56 kDa
Protein length
216 aa Sequence Blast
Gene length
651 bp Sequence Blast
Function
tRNA modification
Product
tRNA:m1A22 methyl transferase
Essential
no
Synonyms
yqfN
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Protein synthesis, modification and degradation → Translation → tRNA modification and maturation
  • Groups of genes → Phosphoproteins → Phosphorylation on a Ser residue

    • Gene

    Coordinates
    2,598,616 → 2,599,266

    The protein

    Catalyzed reaction/ biological activity

  • Catalyzes the S-adenosyl-L-methionine-dependent formation of N1-methyladenine at position 22 (m1A22) in tRNA PubMed
  • adenosine22 in tRNA + S-adenosyl-L-methionine --> H+ + N1-methyladenosine22 in tRNA + S-adenosyl-L-homocysteine PubMed

    • Protein family

  • TrmK family (according to UniProt)

    • Domains

  • N-terminal Class I MTase domain PubMed
  • C-terminal coiled-cloi domain PubMed

    • Modification

  • phosphorylation on Ser-48 PubMed

    • Cofactors

  • SAM PubMed

    • Structure

  • 6Q56 PubMed

    • Localization

  • cytoplasm (according to UniProt)

    • Expression and Regulation

    Operons

    Genes
    trmK-yqfO

    Description

  • PubMed
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    Biological materials

    Mutant

  • BKE25180 (ΔtrmK::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCACCGCTCCATTCGGTA, downstream forward: _UP4_GATCATGGCTAAAAGTGTAA
  • BKK25180 (ΔtrmK::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCACCGCTCCATTCGGTA, downstream forward: _UP4_GATCATGGCTAAAAGTGTAA

    • References

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