trmK [2019-04-02 12:07:01]

tRNA:m1A22 methyl transferase

locus: BSU25180

pI: 5.27

mw: 23.56 kDa

protein length: 216 aa

gene length: 651 bp

function: tRNA modification

product: tRNA:m1A22 methyl transferase

essential: no

synonyms: yqfN

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.5|Phosphorylation on a Ser residue]

Gene

Coordinates: 2,598,616 → 2,599,266

The protein

Catalyzed reaction/ biological activity

• Catalyzes the S-adenosyl-L-methionine-dependent formation of N₁-methyladenine at position 22 (m1A22) in tRNA [pubmed|18420655]
• adenosine²² in tRNA + S-adenosyl-L-methionine --> H⁺ + N¹-methyladenosine²² in tRNA + S-adenosyl-L-homocysteine [pubmed|18420655]

Protein family

• TrmK family (according to UniProt)

[SW|Domains]

• N-terminal Class I MTase domain [pubmed|30931478]
• C-terminal coiled-cloi domain [pubmed|30931478]

Modification

• phosphorylation on Ser-48 [Pubmed|17218307]

[SW|Cofactors]

• SAM [pubmed|30931478]

Structure

• [PDB|6Q56] [pubmed|30931478]

[SW|Localization]

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|C4EF534C1CA613038E1B7042A12EFAA6DA0B5B85|trmK]-[gene|DC60F31B3236C26C919AB7C574F13ABE98E976AF|yqfO]

  description

  • [pubmed|22383849]

Biological materials

Mutant

• BKE25180 (Δ[gene|C4EF534C1CA613038E1B7042A12EFAA6DA0B5B85|trmK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE25180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACCGCTCCATTCGGTA, downstream forward: _UP4_GATCATGGCTAAAAGTGTAA
• BKK25180 (Δ[gene|C4EF534C1CA613038E1B7042A12EFAA6DA0B5B85|trmK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK25180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCACCGCTCCATTCGGTA, downstream forward: _UP4_GATCATGGCTAAAAGTGTAA

References

• 18420655,17218307,27098549,30931478