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cotQ
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cotQ

outer spore coat protein
Locus
BSU_34520
Isoelectric point
6.10
Molecular weight
49.92 kDa
Protein length
447 aa Sequence Blast
Gene length
1344 bp Sequence Blast
Function
protection of the spore
Product
spore coat protein
Essential
no
Synonyms
yvdP
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Lifestyles → Sporulation → Sporulation proteins → Spore coat proteins → Class V

    • Gene

    Coordinates
    3,542,943 → 3,544,286

    The protein

    Protein family

  • oxygen-dependent FAD-linked oxidoreductase family (with YitY and YgaK, according to UniProt)

    • Paralogous protein(s)

  • YgaK:

    • Domains

  • FAD-binding PCMH-type domain (aa 29-201) (according to UniProt)

    • Cofactors

  • FAD (according to UniProt)

    • Structure

  • 3W8W (EncM from Streptomyces maritimus, 32% identity) PubMed

    • Localization

  • outer spore coat, localization depends on CotE PubMed

    • Expression and Regulation

    Operons

    Genes
    cotQ
    Description
    PubMed

    Sigma factors

  • SigK: sigma factor, PubMed, in SigK regulon
  • SigG: sigma factor, PubMed, in SigG regulon

    • Regulatory mechanism

  • GerE: activation, PubMed, in GerE regulon

    • Regulation

  • expressed late during sporulation (SigG, SigK, GerE) PubMed
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    Biological materials

    Mutant

  • MGNA-B620 (yvdP::erm), available at the NBRP B. subtilis, Japan
  • BKE34520 (ΔcotQ::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATATTATTTCACTCCCA, downstream forward: _UP4_TAAAGTTTATTAAAGACAGA
  • BKK34520 (ΔcotQ::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATATTATTTCACTCCCA, downstream forward: _UP4_TAAAGTTTATTAAAGACAGA

    • References

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