cotQ

outer spore coat protein

locus: BSU_34520

pI: 6.10

mw: 49.92 kDa

protein length: 447 aa

gene length: 1344 bp

function: protection of the spore

product: spore coat protein

essential: no

synonyms: yvdP

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.1|Spore coat proteins] → [category|SW 4.2.1.1.5|Class V]

Gene

Coordinates: 3,542,943 → 3,544,286

The protein

Protein family

• oxygen-dependent FAD-linked oxidoreductase family (with [protein|23F2385D14DDD5C400DC897E0F0FC814ACFC36B8|YitY] and [protein|11499CDC836A40E857B8F5AD746BA2C650DC916B|YgaK], according to UniProt)

Paralogous protein(s)

• [protein|11499CDC836A40E857B8F5AD746BA2C650DC916B|YgaK]:

[SW|Domains]

• [SW|FAD-binding PCMH-type domain] (aa 29-201) (according to UniProt)

[SW|Cofactors]

• FAD (according to UniProt)

Structure

• [PDB|3W8W] (EncM from Streptomyces maritimus, 32% identity) [pubmed|24162851]

[SW|Localization]

• outer spore coat, localization depends on [protein|825AD8D4315A85CD384F9AF6AD894E38E57C88F7|CotE] [Pubmed|22171814]

Expression and Regulation

Operons

• genes: [gene|C4CC3772CA9FC46D90D2F15AE261106A0BC4453C|cotQ]
  description: [Pubmed|12480901]

  sigma factors

  • [protein|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK]: sigma factor, [Pubmed|15699190], in [regulon|24F7FD5C7C3A68BB2760ABB8CBD8FBD65E5FF7D4|SigK regulon]
  • [protein|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG]: sigma factor, [Pubmed|12480901], in [regulon|5F24258282F3166B7696B9E9ABC1706E4D06C944|SigG regulon]

  regulatory mechanism

  • [protein|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE]: activation, [Pubmed|15383836], in [regulon|19228BAD44E6DA3DE908DC390FDF628C18D94E65|GerE regulon]

  regulation

  • expressed late during sporulation ([protein|search|SigG], [protein|search|SigK], [protein|search|GerE]) [Pubmed|15383836,15699190,12480901]

Biological materials

Mutant

• MGNA-B620 (yvdP::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1619 NBRP B. subtilis, Japan]
• BKE34520 (Δ[gene|C4CC3772CA9FC46D90D2F15AE261106A0BC4453C|cotQ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE34520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATTATTTCACTCCCA, downstream forward: _UP4_TAAAGTTTATTAAAGACAGA
• BKK34520 (Δ[gene|C4CC3772CA9FC46D90D2F15AE261106A0BC4453C|cotQ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK34520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATATTATTTCACTCCCA, downstream forward: _UP4_TAAAGTTTATTAAAGACAGA

References

• 12562816,15699190,12480901,22171814,15383836,28870294,24162851