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dgcK [2020-03-12 12:03:47]
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dgcK [2020-03-12 12:03:47]

diguanylate cyclase
Locus
BSU_09120
Isoelectric point
8.57
Molecular weight
40.53 kDa
Protein length
359 aa Sequence Blast
Gene length
1080 bp Sequence Blast
Function
synthesis of c-di-GMP
Product
diguanylate cyclase
Essential
no
Synonyms
yhcK
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Nucleotide metabolism → Metabolism of signalling nucleotides
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    985,734 → 986,813

    The protein

    Catalyzed reaction/ biological activity

  • synthesis of c-di-GMP from two molecules of GTP PubMed

    • Domains

  • six transmembrane helices at the N-terminus (according to UniProt?)
  • contains a C-terminal GGDEF domain PubMed
  • GGDEF domain (aa 223-357) (according to UniProt)

    • Structure

  • 2WB4 (the C-terminal GGDEF domain, PleD from Caulobacter vibrioides, 44% identity)

    • Localization

  • cell membrane at cell poles and septa, probably close to YdaK PubMed
  • present at a single site in the cell membrane PubMed

    • Additional information

  • DgcK is present with about 6 molecules per cell PubMed

    • Expression and Regulation

    Operons

    Genes
    dgcK
    Description
    PubMed
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    Biological materials

    Mutant

  • MGNA-A656 (yhcK::erm), available at the NBRP B. subtilis, Japan
  • GP848 (dgcK::ermC), available in Jörg Stülke's lab PubMed
  • BKE09120 (ΔdgcK::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAATATTATCACCCTTATA, downstream forward: _UP4_TGAATTCAATGTTCGAATTC
  • BKK09120 (ΔdgcK::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAATATTATCACCCTTATA, downstream forward: _UP4_TGAATTCAATGTTCGAATTC

    • References

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