SubtiBank SubtiBank
bioW
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

bioW

pimeloyl-CoA synthase (6-carboxyhexanoate-CoA ligase)
Locus
BSU_30240
Isoelectric point
5.55
Molecular weight
29.48 kDa
Protein length
259 aa Sequence Blast
Gene length
777 bp Sequence Blast
Function
biosynthesis of biotin
Product
pimeloyl-CoA synthase
Essential
no
E.C.
6.2.1.14
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
Loading

Categories containing this gene/protein

  • Metabolism → Additional metabolic pathways → Biosynthesis of cofactors → Biosynthesis/ acquisition of biotin

    • Gene

    Coordinates
    3,094,679 → 3,095,455

    Phenotypes of a mutant

  • requires biotin for growth PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • ATP + CoA + heptanedioate --> AMP + diphosphate + heptanedioyl-CoA (according to UniProt)

    • Protein family

  • bioW family (single member, according to UniProt)

    • Structure

  • 5FLG PubMed

    • Expression and Regulation

    Operons

    Genes
    bioW-bioA-bioF-bioD-bioB-bioI-ytbQ
    Description
    PubMed

    Regulatory mechanism

  • BirA: repression, PubMed, in BirA regulon

    • Regulation

  • repressed in the presence of biotin (BirA) PubMed
    • view in new tab

    Biological materials

    Mutant

  • BKE30240 (ΔbioW::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCATTTCTCGCCCTTTCA, downstream forward: _UP4_ATTTTAATAGAGTGGGAGGA
  • BKK30240 (ΔbioW::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCATTTCTCGCCCTTTCA, downstream forward: _UP4_ATTTTAATAGAGTGGGAGGA

    • LacZ fusion

  • pBP627 (in pAC7), available in Fabian Commichau's lab

    • References

    Reviews

    Loading

    Original publications

    Loading