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gudB [Tue Dec 13 2016 11:16:34 GMT+0100 (CET)]
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gudB [Tue Dec 13 2016 11:16:34 GMT+0100 (CET)]

trigger enzyme: glutamate dehydrogenase (cryptic in 168 and derivatives)
locus
BSU22960
pI
5.00
mw
47.00 kDa
function
glutamate utilization, control of GltC activity
product
trigger enzyme: glutamate dehydrogenase
essential
no
ec
1.4.1.2
synonyms
ypcA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.1|Utilization of glutamine/ glutamate]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.7|Control of transcription factor (other than two-component system)]
  • [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.3|Trigger enzyme] → [category|SW 3.4.3.2|Trigger enzymes that control gene expression by protein-protein interaction with transcription factors]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

    • [SW|Categories] containing this gene/protein

  • [SW|utilization of amino acids], [SW|glutamate metabolism], [SW|transcription factors and their control], [SW|trigger enzyme], [SW|phosphoproteins]

    • Gene

    Coordinates on the chromosome (coding sequence)
    2,402,067 -> 2,403,350

    Phenotypes of a mutant

  • The gene is cryptic. If ''gudB'' is activated (''gudB1'' mutation), the bacteria are able to utilize glutamate as the only carbon source. [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=18326565 PubMed]
  • A ''[protein|search|rocG] [protein|search|gudB]'' mutant is sensitive to ß-lactam antibiotics such as cefuroxime and to fosfomycin due to the downregulation of the [SW|SigW regulon] [Pubmed|22178969]
  • transcription profile of a ''[protein|search|rocG] [protein|search|gudB]'' mutant strain: [http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE34383&submit.x=22&submit.y=9 GEO] [Pubmed|22178969]

    • The protein

    Catalyzed reaction/ biological activity

  • L-glutamate H2O NAD = 2-oxoglutarate NH3 NADH H (according to Swiss-Prot)

    • Protein family

  • Glu/Leu/Phe/Val dehydrogenases family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|search|RocG]

    • Modification

  • phosphorylated on Arg-56, Arg-83, and Arg-421 and/or Arg-423 [Pubmed|22517742]

    • [SW|Cofactors]

  • NAD/NADH H

    • Structure

  • [PDB|3K8Z] (enzymatically active GudB1) [Pubmed|20630473]

    • [SW|Interactions]

  • [protein|search|GltC]-[protein|search|GudB] [Pubmed|25711804]

    • Expression and Regulation

    Operon

  • ''gudB'' [Pubmed|22178973]

    • [SW|Sigma factor]

  • [protein|search|SigA] [PubMed|9829940]

    • Regulation

  • constitutively expressed [Pubmed|22178973]

    • Additional information

  • GudB is subject to Clp-dependent proteolysis upon glucose starvation [PubMed|17981983]

    • Biological materials

    Mutant

  • GP691 (''ΔgudB::cat''), GP1160 (''ΔgudB::aphA3'') both available in [SW|Jörg Stülke]'s lab

    • Expression vector

  • for purification of GudB from ''E. coli'' carrying an N-terminal Strep-tag: pGP863 (in [SW|pGP172]) available in [SW|Jörg Stülke]'s lab
  • for purification of GudB1 from ''E. coli'' carrying an N-terminal Strep-tag: pGP864 (in [SW|pGP172]) available in [SW|Jörg Stülke]'s lab
  • for ectopic expression of ''gudB'' with its native promoter: pGP900 (in [protein|search|pAC5]), available in [SW|Jörg Stülke]'s lab
  • wild type ''gudB'', expression in ''B. subtilis'', in [SW|pBQ200]: pGP1712, available in [SW|Jörg Stülke]'s lab
  • pBP179 (N-terminal Strep-tag ''gudB'', purification from ''B. subtilis'', for [SW|SPINE], in [SW|pGP380]), available in [SW|Fabian Commichau]'s lab

    • lacZ fusion

  • pGP651 (in [protein|search|pAC5]), available in [SW|Jörg Stülke]'s lab

    • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW| Fabian Commichau]'s lab

    • FLAG-tag construct

  • GP1194 (''gudB'', ''spc'', based on [SW|pGP1331]), GP1195 (''gudB1'', ''spc'', based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

    • Antibody

  • antibody against [protein|search|RocG] recognizes GudB, available in [SW|Jörg Stülke]'s lab

    • Labs working on this gene/protein

  • [SW|Linc Sonenshein], Tufts University, Boston, MA, USA [http://www.tufts.edu/sackler/microbiology/faculty/sonenshein/index.html Homepage]
  • [SW|Fabian Commichau] University of Göttingen, Germany [http://genmibio.uni-goettingen.de/index.php?id=130 Homepage]

    • References

    Reviews

  • 19698086,8299344,7705101,19895831,22625175

    • Original publications

  • 18603778,9829940,17183217,18723616,18326565,20630473,17981983,21219666,22178973,22517742,23338837,22178969,23785476,24263382,24473333,25610436,25711804