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ycgT
NADPH:ferredoxin oxidoreductase
Molecular weight
36.81 kDa
Product
NADPH:ferredoxin oxidoreductase
Genomic Context
Categories containing this gene/protein
Gene
Coordinates
352,858 → 353,868
The protein
Catalyzed reaction/ biological activity
H+ + NADP+ + 2 reduced [2Fe-2S]-[ferredoxin] --> NADPH + 2 oxidized [2Fe-2S]-[ferredoxin] (according to UniProt)Protein family
ferredoxin--NADP reductase type 2 family (with YumC, according to UniProt) Paralogous protein(s)
Cofactors
Structure
Additional information
The gene is annotated in KEGG as an ortholog of thioredoxin reductase (NADPH) EC 1.8.1.9. In MetaCyc the protein is marked as “similar to thioredoxinreductase”. No EC annotation is available in Swiss-Prot. No literature/experimental evidence supporting the annotation is available. PubMed Expression and Regulation
Operons
Regulatory mechanism
Regulation
induced under conditions of iron starvation (Fur) PubMed Additional information
the mRNA is very stable (half-life > 15 min) PubMed view in new tabBiological materials
Mutant
MGNA-C052 (ycgT::erm), available at the NBRP B. subtilis, JapanGP1852 (ΔycgT::cat), available in Jörg Stülke's labBKE03270 (ΔycgT::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCGTCCTGCCTCCCGTT, downstream forward: _UP4_TAAAACAGCCCTTGAGGAAABKK03270 (ΔycgT::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCGTCCTGCCTCCCGTT, downstream forward: _UP4_TAAAACAGCCCTTGAGGAAA References
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