ycgT

NADPH:ferredoxin oxidoreductase

Locus: BSU_03270

Isoelectric point: 5.61

Molecular weight: 36.81 kDa

Protein length: 336 aa

Gene length: 1011 bp

Function: unknown

Product: NADPH:ferredoxin oxidoreductase

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Electron transport and ATP synthesis → Electron transport/ other/ based on similarity

Gene

Coordinates: 352,858 → 353,868

The protein

Catalyzed reaction/ biological activity

• H⁺ + NADP⁺ + 2 reduced [2Fe-2S]-[ferredoxin] --> NADPH + 2 oxidized [2Fe-2S]-[ferredoxin] (according to UniProt)

Protein family

• ferredoxin--NADP reductase type 2 family (with YumC, according to UniProt)

Paralogous protein(s)

• YumC

Cofactors

• FAD PubMed

Structure

• 5XHU

Additional information

• The gene is annotated in KEGG as an ortholog of thioredoxin reductase (NADPH) EC 1.8.1.9. In MetaCyc the protein is marked as “similar to thioredoxin
• reductase”. No EC annotation is available in Swiss-Prot. No literature/experimental evidence supporting the annotation is available. PubMed

Expression and Regulation

Operons

• Genes: ycgT
  Description: PubMed

  Regulatory mechanism

  • Fur: repression, PubMed, in Fur regulon

  Regulation

  • induced under conditions of iron starvation (Fur) PubMed

  Additional information

  • the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

Mutant

• MGNA-C052 (ycgT::erm), available at the NBRP B. subtilis, Japan
• GP1852 (ΔycgT::cat), available in Jörg Stülke's lab
• BKE03270 (ΔycgT::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCGTCCTGCCTCCCGTT, downstream forward: _UP4_TAAAACAGCCCTTGAGGAAA
• BKK03270 (ΔycgT::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCGTCCTGCCTCCCGTT, downstream forward: _UP4_TAAAACAGCCCTTGAGGAAA

References

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