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ycgT
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ycgT

NADPH:ferredoxin oxidoreductase
Locus
BSU_03270
Isoelectric point
5.61
Molecular weight
36.81 kDa
Protein length
336 aa Sequence Blast
Gene length
1011 bp Sequence Blast
Function
unknown
Product
NADPH:ferredoxin oxidoreductase
Essential
no
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
352,858 → 353,868

The protein

Catalyzed reaction/ biological activity

  • H+ + NADP+ + 2 reduced [2Fe-2S]-[ferredoxin] --> NADPH + 2 oxidized [2Fe-2S]-[ferredoxin] (according to UniProt)
  • Protein family

  • ferredoxin--NADP reductase type 2 family (with YumC, according to UniProt)
  • Paralogous protein(s)

    Cofactors

  • FAD PubMed
  • Structure

    Additional information

  • The gene is annotated in KEGG as an ortholog of thioredoxin reductase (NADPH) EC 1.8.1.9. In MetaCyc the protein is marked as “similar to thioredoxin
  • reductase”. No EC annotation is available in Swiss-Prot. No literature/experimental evidence supporting the annotation is available. PubMed
  • Expression and Regulation

    Operons

    Genes
    Description

    Regulatory mechanism

  • Fur: repression, PubMed, in Fur regulon
  • Regulation

  • induced under conditions of iron starvation (Fur) PubMed
  • Additional information

  • the mRNA is very stable (half-life > 15 min) PubMed
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    Biological materials

    Mutant

  • MGNA-C052 (ycgT::erm), available at the NBRP B. subtilis, Japan
  • GP1852 (ΔycgT::cat), available in Jörg Stülke's lab
  • BKE03270 (ΔycgT::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCGTCCTGCCTCCCGTT, downstream forward: _UP4_TAAAACAGCCCTTGAGGAAA
  • BKK03270 (ΔycgT::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCGTCCTGCCTCCCGTT, downstream forward: _UP4_TAAAACAGCCCTTGAGGAAA
  • References

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