iolE [Tue Dec 13 2016 11:16:37 GMT+0100 (CET)]

2-keto-myo-inositol dehydratase, dehydration of 2-keto-myo-inositol (2nd reaction)

Locus: BSU39720

Isoelectric point: 5.21

Molecular weight: 33.00 kDa

Function: myo-inositol catabolism

Product: 2-keto-myo-inositol dehydratase,
dehydration of 2-keto-myo-inositol (2nd reaction)

Essential: no

E.C.: 4.2.1.44

Synonyms: yxdE

Genomic Context

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Categories containing this gene/protein

• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of inositol
• Groups of genes → Phosphoproteins → Phosphorylation on an Arg residue

Categories containing this gene/protein

• utilization of specific carbon sources

This gene is a member of the following regulons

• CcpA regulon, IolR regulon

Gene

Coordinates on the chromosome (coding sequence): 4,078,173 -> 4,079,066

The protein

Catalyzed reaction/ biological activity

• 2,4,6/3,5-pentahydroxycyclohexanone = 3,5/4-trihydroxycyclohexa-1,2-dione + H₂O (according to Swiss-Prot)

Protein family

• iolE/mocC family (according to Swiss-Prot)

Structure

• 3CNY (from Lactobacillus plantarum wcfs1 mutant, 63% identity, 78% similarity)

Expression and Regulation

Operon

• iolA-iolB-iolC-iolD-iolE-iolF-iolG-iolH-iolI-iolJ PubMed

Sigma factor

• SigA PubMed

Regulation

• repressed by glucose (CcpA) PubMed
• induced by inositol (IolR) PubMed

Regulatory mechanism

• IolR: transcription repression PubMed
• CcpA: transcription repression PubMed

Labs working on this gene/protein

• Yasutaro Fujita, University of Fukuyama, Japan
• [[Ken-ichi Yoshida]], Kobe University, Japan

References

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