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pucF [2017-8-11 16:0:3]
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pucF [2017-8-11 16:0:3]

allantoate amidohydrolase
locus
BSU32530
pI
5.36
mw
45.36 kDa
protein length
412 aa Sequence Blast
gene length
1236 bp Sequence Blast
function
purine utilization
product
allantoate amidohydrolase
essential
no
synonyms
yurH
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.1|Utilization of nucleotides]

    • Gene

    Coordinates
    3,342,433 → 3,343,671

    The protein

    Protein family

  • peptidase M20 family (according to Swiss-Prot)

    • Structure

  • [PDB|2IMO] (from ''Escherichia coli k12'', 47% identity, 63% similarity) [Pubmed|17362992]

    • Expression and Regulation

    Operons

    genes
    [gene|C314CE9D194E8894430368C5F69C0778D45A651C|pucF]-[gene|4BC4B3E0AB4779D1DD5EDF591361FC1094AF661F|pucG]
    description
    [Pubmed|12029039]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12029039], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|52C1601482C26400A524E880334BB801F832D6ED|PucR]: activation, [Pubmed|12029039], in [regulon|52C1601482C26400A524E880334BB801F832D6ED|PucR regulon]

    • regulation

  • induced in the presence of purine nucleotides (inducer: allantoin) ([protein|search|PucR]) [Pubmed|12029039]
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    Biological materials

    Mutant

  • MGNA-A943 (yurH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/943 NBRP B. subtilis, Japan]

    • References

  • 11344136,12029039