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ydeF [2019-08-28 08:56:31]
similar to transcriptional regulator ([SW|MocR/ GabR family])
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.6|Transcription factor/ other/ based on similarity]Gene
Coordinates
564,704 → 566,101
The protein
Protein family
[SW|MocR/ GabR family] [Pubmed|22020104][SW|class-I pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)[SW|Domains]
N-terminal DNA-binding helix-turn-helix motif; C-terminal domain is homologous to PLP-binding large domain of aminotransferases.[SW|Cofactors]
PLPStructure
[PDB|4MGR] ([protein|C7C36FAC0CE72226960FC7E8E016B8EC77AF1036|GabR], 23% identity) [pubmed|24127574]Biological materials
Mutant
MGNA-C082 (ydeF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2080 NBRP B. subtilis, Japan]BKE05180 (Δ[gene|C2EB2BE786A5C155DE440E52CB430B25E70C8AA7|ydeF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE05180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATACGCATCCTTTTTC, downstream forward: _UP4_TAATATTGTTTGCCATACAABKK05180 (Δ[gene|C2EB2BE786A5C155DE440E52CB430B25E70C8AA7|ydeF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK05180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATACGCATCCTTTTTC, downstream forward: _UP4_TAATATTGTTTGCCATACAAReferences
11756427,22020104,24127574