ydeF

similar to transcriptional regulator ([SW|MocR/ GabR family])

locus: BSU_05180

pI: 6.09

mw: 51.64 kDa

protein length: 465 aa

gene length: 1398 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.6|Transcription factor/ other/ based on similarity]

Gene

Coordinates: 564,704 → 566,101

The protein

Protein family

• [SW|MocR/ GabR family] [Pubmed|22020104]
• [SW|class-I pyridoxal-phosphate-dependent aminotransferase family] (according to UniProt)

[SW|Domains]

• N-terminal DNA-binding helix-turn-helix motif; C-terminal domain is homologous to PLP-binding large domain of aminotransferases.
• [SW|HTH gntR-type domain] (aa 22-90) (according to UniProt)

[SW|Cofactors]

• PLP

Structure

• [PDB|4MGR] ([protein|C7C36FAC0CE72226960FC7E8E016B8EC77AF1036|GabR], 23% identity) [pubmed|24127574]

Biological materials

Mutant

• MGNA-C082 (ydeF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2080 NBRP B. subtilis, Japan]
• BKE05180 (Δ[gene|C2EB2BE786A5C155DE440E52CB430B25E70C8AA7|ydeF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE05180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATACGCATCCTTTTTC, downstream forward: _UP4_TAATATTGTTTGCCATACAA
• BKK05180 (Δ[gene|C2EB2BE786A5C155DE440E52CB430B25E70C8AA7|ydeF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK05180 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATACGCATCCTTTTTC, downstream forward: _UP4_TAATATTGTTTGCCATACAA

References

• 11756427,22020104,24127574