yflK [2018-07-26 15:58:58]

unknown

locus: BSU07650

pI: 7.76

mw: 24.69 kDa

protein length: 221 aa

gene length: 663 bp

function: unknown

product: unknown

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.7|Detoxification reactions]

Gene

Coordinates: 838,077 → 838,742

The protein

Structure

• [PDB|5YHH] (from Geobacillus stearothermophilus, 50% identity) [pubmed|29459651]

Expression and Regulation

Operons

• genes: [gene|B51ADFB53CA54309D866BB089502639E9571238B|nos]-[gene|C2E28821689A07B9AA1B0BD5C84D856C04D83E95|yflK]
  description: [Pubmed|22383849]

  additional information

  • Note that the [gene|D7C46835B9984E2C5E375816F2BB16566202CF27|yflL] is located between [gene|B51ADFB53CA54309D866BB089502639E9571238B|nos] and [gene|C2E28821689A07B9AA1B0BD5C84D856C04D83E95|yflK], but on the opposite strand

Biological materials

Mutant

• MGNA-C335 (yflK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2333 NBRP B. subtilis, Japan]
• BKE07650 (Δ[gene|C2E28821689A07B9AA1B0BD5C84D856C04D83E95|yflK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07650 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACAAACACATCCCCTTT, downstream forward: _UP4_TAGAAAAAAGCCGCGCATAT
• BKK07650 (Δ[gene|C2E28821689A07B9AA1B0BD5C84D856C04D83E95|yflK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07650 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACAAACACATCCCCTTT, downstream forward: _UP4_TAGAAAAAAGCCGCGCATAT

References

• 11886751,29459651