SubtiBank SubtiBank
pyrK
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pyrK

dihydroorotic acid dehydrogenase (electron transfer subunit)
Locus
BSU_15530
Isoelectric point
5.58
Molecular weight
27.95 kDa
Protein length
256 aa Sequence Blast
Gene length
771 bp Sequence Blast
Function
pyrimidine biosynthesis
Product
dihydroorotic acid dehydrogenase (electron transfer subunit)
Essential
no
Synonyms
pyrDII,ylxD
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Nucleotide metabolism → Biosynthesis/ acquisition of nucleotides → Biosynthesis/ acquisition of pyrimidine nucleotides

    • Gene

    Coordinates
    1,626,948 → 1,627,718

    The protein

    Protein family

  • PyrK family (single member, according to UniProt)

    • Domains

  • FAD-binding FR-type domain (aa 1-101) (according to UniProt)

    • Cofactors

  • [2Fe-2S] cluster PubMed
  • FAD PubMed

    • Structure

  • 1EP1 (from Lactococcus lactis, 46% identity) PubMed

    • Additional information

  • belongs to the 100 most abundant proteins PubMed

    • Expression and Regulation

    Operons

    Genes
    pyrR-pyrP-pyrB-pyrC-pyrAA-pyrAB-pyrK-pyrD-pyrF-pyrE
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • PyrR: termination/ antitermination, via RNA switch, in PyrR regulon

    • Regulation

  • induced in the absence of uridine nucleotides (PyrR) PubMed
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    Biological materials

    Mutant

  • BKE15530 (ΔpyrK::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ACATACTGTCAAATACGCTT, downstream forward: _UP4_AAAGCTCAGGAGGTGGCGCT
  • BKK15530 (ΔpyrK::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_ACATACTGTCAAATACGCTT, downstream forward: _UP4_AAAGCTCAGGAGGTGGCGCT

    • References

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