araN

[SW|ABC transporter] for the uptake of α-1,5-arabinooligosaccharides, at least up to four L-arabinosyl units (sugar-binding protein)

locus: BSU_28750

pI: 8.56

mw: 48.48 kDa

protein length: 433 aa

gene length: 1302 bp

function: uptake of α-1,5-arabinooligosaccharides

product: α-1,5-arabinooligosaccharide [SW|ABC transporter ](sugar-binding protein)

essential: no

synonyms: yseC

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.1|Uptake of carbon sources]
• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.6|Utilization of arabinan/ arabinose/ arabitol]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 2,941,674 → 2,942,975

The protein

Protein family

• [SW|Bacterial solute-binding protein 1 family] (according to UniProt)

Structure

• [PDB|5YSB] (from Listeria innocua, 28% identity) [pubmed|29678880]

[SW|Localization]

• cell membrane (via [protein|8AF43CA410F3381209196E1B9A4FE66D78020793|AraP]-[protein|AE532A8931D516CC676E765D1D1293E73C335D23|AraQ]) [Pubmed|10092453]

Expression and Regulation

Operons

• genes: [gene|028428CE456E283C065756878EA7C7DB495DD10D|araA]-[gene|E0525A4187D9C8A23F23BC8F883973896C8D295D|araB]-[gene|3859854178E573AE8ACCE6DF85E32C495D316DA1|araD]-[gene|BBAD1638F435791C1846EFFAD6B6F82CE491FDF1|araL]-[gene|16EE17461B4AB467A4F8172FD3E335073AD2B1D8|araM]-[gene|C1F5D0EA21DCA5F8EC43720CDF67E859CA7B866E|araN]-[gene|8AF43CA410F3381209196E1B9A4FE66D78020793|araP]-[gene|AE532A8931D516CC676E765D1D1293E73C335D23|araQ]-[gene|2B2629D275491A9F7031D5A99BAA001B011A765B|abfA]
  description: [Pubmed|9084180]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|9084180], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|12949161], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]
  • [protein|A567466894AE9DE7CDE7816615433A37532297B5|AraR]: repression, [Pubmed|10417639], in [regulon|A567466894AE9DE7CDE7816615433A37532297B5|AraR regulon]

  regulation

  • induced by arabinose ([protein|search|AraR]) [Pubmed|10417639]

  additional information

  • the mRNA is very stable (half-life > 15 min) [http://www.ncbi.nlm.nih.gov/sites/entrez/12884008 PubMed]

Biological materials

Mutant

• MGNA-B004 (araN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1003 NBRP B. subtilis, Japan]
• BKE28750 (Δ[gene|C1F5D0EA21DCA5F8EC43720CDF67E859CA7B866E|araN]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE28750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTGCGTTACCCCTTCCA, downstream forward: _UP4_TAGAATCCCATTCAAAAAGT
• BKK28750 (Δ[gene|C1F5D0EA21DCA5F8EC43720CDF67E859CA7B866E|araN]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK28750 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTGCGTTACCCCTTCCA, downstream forward: _UP4_TAGAATCCCATTCAAAAAGT

References

• 10092453,10417639,9084180,20693325,29678880