SubtiBank SubtiBank
gapB [Tue Dec 13 2016 11:16:35 GMT+0100 (CET)]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

gapB [Tue Dec 13 2016 11:16:35 GMT+0100 (CET)]

glyceraldehyde-3-phosphate dehydrogenase, NADP-dependent, gluconeogenic enzyme, forms a transhydrogenation cycle with GapA for balancing of NADPH
locus
BSU29020
pI
6.00
mw
37.00 kDa
function
anabolic enzyme in gluconeogenesis
product
glyceraldehyde-3-phosphate dehydrogenase 2
essential
no
ec
1.2.1.59
synonyms
ppc
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.2|Gluconeogenesis]

    • [SW|Categories] containing this gene/protein

  • [SW|carbon core metabolism], [SW|sporulation/ other]

    • This gene is a member of the following [SW|regulons]

  • [SW|CcpN regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    2,967,032 -> 2,968,054

    Phenotypes of a mutant

  • inactivation of ''[SW|gapB]'' reduces sporulation efficiency to 0.3% that of wild type cells; delayed entry into sporulation and fewer sporulating cells [Pubmed|26735940]

    • The protein

    Catalyzed reaction/ biological activity

  • D-glyceraldehyde 3-phosphate phosphate NAD(P) = 3-phospho-D-glyceroyl phosphate NAD(P)H (according to Swiss-Prot)
  • This reaction is part of the gluconeogenesis

    • Protein family

  • glyceraldehyde-3-phosphate dehydrogenase family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|search|GapA]

    • Kinetic information

  • Michaelis-Menten [Pubmed|10799476]

    • [SW|Domains]

  • Nucleotid bindinge domain (12-13)
  • 2x Glyceraldehyde 3-phosphate binding domain (151-153) & (210-211)

    • [SW|Cofactors]

  • NADP (preferentially) and NAD [Pubmed|10799476]

    • Structure

  • [PDB|3PRL] (from ''B. halodurans'')

    • [SW|Localization]

  • Cytoplasm (Homogeneous) [Pubmed|16479537]

    • Expression and Regulation

    Operon

  • ''[protein|search|gapB]-[protein|search|speD]'' [Pubmed|15720552]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|15720552]

    • Regulation

  • repressed in the presence of glucose (70-fold) ([protein|search|CcpN]) [Pubmed|15720552]
  • strongly induced in response to glucose starvation in M9 medium [Pubmed|23033921]

    • Regulatory mechanism

  • [protein|search|CcpN]: transcription repression [Pubmed|15720552]

    • Biological materials

    Mutant

  • GP701 (''gapB''::''spec''), available in [SW|Stülke] lab
  • 1A1004 ( ''gapB''::''erm''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A1004&Search=1A1004 BGSC]

    • Labs working on this gene/protein

  • [SW|Stephane Aymerich], Microbiology and Molecular Genetics, INRA Paris-Grignon, France

    • References

  • 16479537,10799476,10844697,15720552,18586936,17114254,22190493,23033921,22740702,26735940