motI [2019-09-18 09:48:16]

c-di-GMP receptor protein, inhibits flagellar rotation at high c-di-GMP concentrations

Locus: BSU_22910

Isoelectric point: 7.88

Molecular weight: 24.98 kDa

Protein length: 217 aa

Gene length: 654 bp

Function: control of MotA activity

Product: c-di-GMP receptor protein

Essential: no

Synonyms: dgrA,ypfA,jofA

Genomic Context

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Categories containing this gene/protein

• Information processing → Targets of second messengers → Targets of c-di-GMP
• Lifestyles → Exponential and early post-exponential lifestyles → Motility and chemotaxis → Signal transduction in motility and chemotaxis → Additional chemotaxis signal transduction and regulatory proteins

Gene

Coordinates: 2,397,019 → 2,397,672

The protein

Domains

• contains a C-terminal PilZ domain PubMed

Cofactors

• c-di-GMP PubMed

Effectors of protein activity

• binding of c-di-GMP stimulates the inhibitory interaction with MotA PubMed

Structure

• 5VX6 (complex with c-di-GMP) PubMed

Localization

• forms MotA-dependent spots at the membrane PubMed

Expression and Regulation

Operons

• Genes: motI-ypfB-cmk-ypfD-fni
  Description: PubMed

• Genes: motI-ypfB-cmk-ypfD
  Description: PubMed

Biological materials

Mutant

• MGNA-A419 (ypfA::erm), available at the NBRP B. subtilis, Japan
• BKE22910 (ΔmotI::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTGACTTCACTCTTTCGC, downstream forward: _UP4_TAAACATCGGCAAACACTTC
• BKK22910 (ΔmotI::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTGACTTCACTCTTTCGC, downstream forward: _UP4_TAAACATCGGCAAACACTTC

References

Reviews

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Original Publications

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