yitS

fatty acid binding protein

locus: BSU_11110

pI: 5.63

mw: 31.12 kDa

protein length: 283 aa

gene length: 852 bp

function: phosphorylation of fatty acids

product: fatty acid binding protein

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.2|Biosynthesis of phospholipids]

Gene

Coordinates: 1,187,700 → 1,188,551

The protein

Catalyzed reaction/ biological activity

• binds and presents fatty acids for phosphorylation to [protein|5DE73E8005AFFD30EB84696B2C2474899C6D1982|FakA] (based on findings for homologous proteins from Staphylococcus aureus) [pubmed|30429218]

Paralogous protein(s)

• [protein|FB9ABF1FE4EC062C039A62B28A192BAA9B565EEF|DegV]

[SW|Domains]

• DegV domain (aa 3-282) (according to UniProt)

Structure

• [PDB|1PZX] (Geobacillus stearothermophilus)

Expression and Regulation

Operons

• genes: [gene|C13F1870B014F8B2C16D9B169F40747DE5B51B85|yitS]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B196 (yitS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1195 NBRP B. subtilis, Japan]
• BKE11110 (Δ[gene|C13F1870B014F8B2C16D9B169F40747DE5B51B85|yitS]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE11110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTGCTTCCCTTCTTTC, downstream forward: _UP4_TGAGCAAACAAAAACAGTCA
• BKK11110 (Δ[gene|C13F1870B014F8B2C16D9B169F40747DE5B51B85|yitS]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK11110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATTGCTTCCCTTCTTTC, downstream forward: _UP4_TGAGCAAACAAAAACAGTCA

References

Research papers

• 30429218