yyaF [Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)]

GTP-binding protein/ GTPase

locus: BSU40920

pI: 4.66

mw: 39.00 kDa

function: unknown

product: GTP-binding protein/ GTPase

essential: no

synonyms

Genomic Context

categories

• [category|SW 6|Groups of genes] → [category|SW 6.3|GTP-binding proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.7|Proteins of unknown function]

[SW|Categories] containing this gene/protein

• [SW|GTP-binding proteins], [SW|proteins of unknown function]

This gene is a member of the following [SW|regulons]

• [SW|ComK regulon]

Gene

Coordinates on the chromosome (coding sequence): 4,199,843 -> 4,200,943

The protein

Catalyzed reaction/ biological activity

• Binds and hydrolyzes GTP and readily exchanges GDP for GTP

Protein family

• EngD subfamily (according to Swiss-Prot) Era/Obg family

Paralogous protein(s)

• [protein|search|Era], [protein|search|Obg], [protein|search|YphC], [protein|search|YsxC], [protein|search|RbgA], [protein|search|YqeH], [protein|search|ThdF], [protein|search|YyaF], [protein|search|YnbA]

Expression and Regulation

Operon

• ''[protein|search|yyaF]-[protein|search|rpsF]-[protein|search|ssbA]-[protein|search|rpsR]'' [Pubmed|14762004]

Regulation

• [protein|search|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
• [protein|search|ComK]: transcription activation [Pubmed|11948146]

Biological materials

Expression vector

• for expression/ purification from ''B. subtilis'' with N-terminal Strep-tag, for [SW|SPINE], in [SW|pGP380]: pGP836, available in [SW|Stülke] lab
• for expression/ purification from ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP844, available in [SW|Stülke] lab

Labs working on this gene/protein

• [SW|Naotake Ogasawara], Nara, Japan

References

Reviews

• 21885683

Original publications

• 12427945,14762004,11948165,11948146