yyaF

GTP-binding protein/ GTPase

Locus: BSU_40920

Isoelectric point: 4.66

Molecular weight: 39.91 kDa

Protein length: 366 aa

Gene length: 1101 bp

Function: unknown

Product: GTP-binding protein/ GTPase

Essential: no

Synonyms: engD

Genomic Context

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Categories containing this gene/protein

• Groups of genes → GTP-binding proteins
• Groups of genes → Proteins of unknown function

Gene

Coordinates: 4,199,843 → 4,200,943

The protein

Catalyzed reaction/ biological activity

• Binds and hydrolyzes GTP and readily exchanges GDP for GTP

Protein family

• TRAFAC class OBG-HflX-like GTPase superfamily (with Obg and YnbA, according to UniProt)

Paralogous protein(s)

• Era, YsxC, RbgA, ThdF, Obg, YqeH, YnbA, YphC

Domains

• OBG-typeG domain (aa 3-259) (according to UniProt)
• TGS domain (aa 281-364) (according to UniProt)

Structure

• 1JAL (from Haemophilus influenzae, 58% identity) PubMed

Expression and Regulation

Operons

• Genes: yyzM-yyaE-yyaF-rpsF-ssbA-rpsR
  Description: PubMed

• Genes: yyaF-rpsF-ssbA-rpsR
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • ComK: activation, in ComK regulon
  • stringent response: negative regulation, in stringent response

  Regulation

  • ComK: transcription activation PubMed

Biological materials

Mutant

• MGNA-B869 (yyaF::erm), available at the NBRP B. subtilis, Japan
• BKE40920 (ΔyyaF::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCTTTCATCTCCTCTAT, downstream forward: _UP4_TAGGATGCAGTTGTAAAGGG
• BKK40920 (ΔyyaF::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCTTTCATCTCCTCTAT, downstream forward: _UP4_TAGGATGCAGTTGTAAAGGG

Expression vectors

• for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP836, available in Stülke lab
• for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP844, available in Stülke lab

Labs working on this gene/protein

• Naotake Ogasawara, Nara, Japan

References

Reviews

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Original publications

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