citM

Mg²⁺-citrate transporter

locus: BSU_07610

pI: 6.23

mw: 45.67 kDa

protein length: 433 aa

gene length: 1302 bp

function: uptake of citrate and magnesium

product: Mg²⁺-citrate transporter

essential: no

synonyms: yflO

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.4|Transporter for organic acids]
• [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.1|Metal ion homeostasis (K, Na, Ca, Mg)] → [category|SW 1.3.1.1|Magnesium uptake/ efflux]
• [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.1|Utilization of organic acids]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 834,383 → 835,684

Phenotypes of a mutant

• no growth with citrate as sole carbon source [Pubmed|10972810]

The protein

Protein family

• [SW|CitM transporter family (TC 2.A.11)] (according to UniProt)

Paralogous protein(s)

• [protein|71A73C825981E74842FC28D1B05CF85E9F3F5A56|CitH]

[SW|Localization]

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|C0DED68802EF78D2BC16507234AAC3B60D236E68|citM]-[gene|66B9809A77AB15E5C8C8E5A8E26752771AF55E3A|yflN]
  description: [Pubmed|10972810]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|10972810], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|1345FBA88CB55E0E897FEC8DA4D829B85A8393BB|CitT]: activation, [Pubmed|10972810], in [regulon|1345FBA88CB55E0E897FEC8DA4D829B85A8393BB|CitT regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|22900538,10972810], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

  regulation

  • induced by citrate ([protein|search|CitT]) [Pubmed|10972810]

Biological materials

Mutant

• MGNA-C252 (citM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2250 NBRP B. subtilis, Japan]
• GP2244 (''Δ''''[gene|C0DED68802EF78D2BC16507234AAC3B60D236E68|citM]''::''ermC''), available in [SW|Jörg Stülke]'s lab
• GP2760 (''Δ''''[gene|C0DED68802EF78D2BC16507234AAC3B60D236E68|citM]''::''kan''), available in [SW|Jörg Stülke]'s lab
• GP2761 (''Δ''''[gene|C0DED68802EF78D2BC16507234AAC3B60D236E68|citM]''::''spec''), available in [SW|Jörg Stülke]'s lab
• BKE07610 (Δ[gene|C0DED68802EF78D2BC16507234AAC3B60D236E68|citM]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACATCCATTCCCCCTTAAA, downstream forward: _UP4_TGATAATTCAGGCGTAATGA
• BKK07610 (Δ[gene|C0DED68802EF78D2BC16507234AAC3B60D236E68|citM]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07610 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACATCCATTCCCCCTTAAA, downstream forward: _UP4_TGATAATTCAGGCGTAATGA
• GP2763 (''Δ''''[gene|C0DED68802EF78D2BC16507234AAC3B60D236E68|citM]''::''kan'' ''Δ''''[gene|472E3A2407C83EEE26DB00079D185C4EA2988611|mgtE]''::''spec''), available in [SW|Jörg Stülke]'s lab

lacZ fusion

• pGP2965 (in [SW|pAC6]), available in [SW|Jörg Stülke]'s lab

FLAG-tag construct

• GP2767 ''citM-3xFLAG spc'' (based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

References

• 16842348,11891560,10972810,11053381,3127276,12375105,8892821,12533460,12670692,11029430,12427932,22900538,24415722