yvaK [2019-08-09 12:24:39]

general stress protein, carboxylesterase

locus: BSU_33620

pI: 4.99

mw: 28.26 kDa

protein length: 248 aa

gene length: 747 bp

function: unknown

product: unknown

essential: no

ec: 3.1.1.1

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.1|General stress proteins (controlled by SigB)]
• [category|SW 6|Groups of genes] → [category|SW 6.6|Poorly characterized/ putative enzymes]

Gene

Coordinates: 3,454,221 → 3,454,967

The protein

Catalyzed reaction/ biological activity

• A carboxylic ester + H₂O = an alcohol + a carboxylate (according to Swiss-Prot)

Protein family

• lipase/esterase LIP3/BchO family (single member, according to UniProt)

Structure

• [PDB|1TQH] (Geobacillus stearothermophilus)

Expression and Regulation

Operons

• genes: [gene|D8851F453E5EF18D36F25834A8CB9C61EAF15E44|secG]-[gene|C0B6520E74CE9F7EE3DEC8C0D83E0770F241C4E8|yvaK]-[gene|0472A4346ADA1DC41C9D00E8F3222A0EBB76DFA8|rnr]-[gene|D19519B5A776D6E8EC2BFE7A7D67469CA0A6459A|smpB]-[gene|5C12BB19B975F7FEBCFA119DAC66338C20FCECFA|ssrA]
  description: [Pubmed|17369301]

  sigma factors

  • [protein|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB]: sigma factor, [Pubmed|11544224], in [regulon|580011DE5DC40EC9E7E0512791D328FAA010DCB8|SigB regulon]

  regulation

  • ''[protein|search|yvaK]'': induced by stress ([protein|search|SigB]) [Pubmed|11544224]

• genes: [gene|C0B6520E74CE9F7EE3DEC8C0D83E0770F241C4E8|yvaK]-[gene|0472A4346ADA1DC41C9D00E8F3222A0EBB76DFA8|rnr]-[gene|D19519B5A776D6E8EC2BFE7A7D67469CA0A6459A|smpB]-[gene|5C12BB19B975F7FEBCFA119DAC66338C20FCECFA|ssrA]
  description: [Pubmed|17369301]

  regulation

  • ''[protein|search|yvaK]'': induced by stress ([protein|search|SigB]) [Pubmed|11544224]

Biological materials

Mutant

• MGNA-B606 (yvaK::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1605 NBRP B. subtilis, Japan]
• BKE33620 (Δ[gene|C0B6520E74CE9F7EE3DEC8C0D83E0770F241C4E8|yvaK]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE33620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTCATGTCTCCCTTTC, downstream forward: _UP4_TGGTAATCAACAGGAGGTCG
• BKK33620 (Δ[gene|C0B6520E74CE9F7EE3DEC8C0D83E0770F241C4E8|yvaK]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK33620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCTCATGTCTCCCTTTC, downstream forward: _UP4_TGGTAATCAACAGGAGGTCG

References

• 11544224,17369301,23812333