yqgT

similar to gamma-D-glutamyl-L-diamino acid endopeptidase I

Locus: BSU_24830

Isoelectric point: 5.91

Molecular weight: 43.28 kDa

Protein length: 376 aa

Gene length: 1131 bp

Function: cell wall metabolism

Product: peptidoglycan hydrolase

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Cell envelope and cell division → Cell wall degradation/ turnover → Endopeptidases
• Cellular processes → Cell envelope and cell division → Cell wall/ other

Gene

Coordinates: 2,567,360 → 2,568,490

The protein

Catalyzed reaction/ biological activity

• cleaves the peptide bond between D-Glu (position 2 in the peptioglycan peptide) and m-diamino pimelic acid (position 3) PubMed
• has both endopeptidase and carboxypeptidase activities PubMed

Protein family

• peptidase M14 family (single member, according to UniProt)

Cofactors

• Zn

Localization

• extracellular space (according to UniProt)

Expression and Regulation

Operons

• Genes: yqgT-yqgU-yqgV
  Description: PubMed

Biological materials

Mutant

• MGNA-C480 (yqgT::erm), available at the NBRP B. subtilis, Japan
• BKE24830 (ΔyqgT::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCATCCCCCTTTTTTGTT, downstream forward: _UP4_TAACTGAAACTTTTTCTTTT
• BKK24830 (ΔyqgT::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCATCCCCCTTTTTTGTT, downstream forward: _UP4_TAACTGAAACTTTTTCTTTT

References

Reviews

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Original publications

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