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purT [2019-07-22 09:43:58]
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purT [2019-07-22 09:43:58]

phosphoribosylglycinamide formyltransferase 2
Locus
BSU_02230
Isoelectric point
4.72
Molecular weight
41.93 kDa
Protein length
384 aa Sequence Blast
Gene length
1155 bp Sequence Blast
Function
purine biosynthesis
Product
phosphoribosylglycinamide formyltransferase 2
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Nucleotide metabolism → Biosynthesis/ acquisition of nucleotides → Biosynthesis/ acquisition of purine nucleotides

    • Gene

    Coordinates
    243,892 → 245,046

    The protein

    Catalyzed reaction/ biological activity

  • Formate + ATP + 5'-phospho-ribosylglycinamide = 5'-phosphoribosyl-N-formylglycinamide + ADP + diphosphate (according to Swiss-Prot)

    • Protein family

  • PurK/PurT family (with PurK, according to UniProt)

    • Paralogous protein(s)

  • PurK

    • Structure

  • 1KJ9 (with Mg-ATP from Escherichia coli, 55% identity, 69% similarity) PubMed

    • Expression and Regulation

    Operons

    Genes
    purT
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
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    Biological materials

    Mutant

  • BKE02230 (ΔpurT::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTTTGCCCCTCCTATCT, downstream forward: _UP4_TAGAGTTTGAACAGGTCTTG
  • BKK02230 (ΔpurT::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTTTGCCCCTCCTATCT, downstream forward: _UP4_TAGAGTTTGAACAGGTCTTG

    • References

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