purT [2019-07-22 09:43:58]

phosphoribosylglycinamide formyltransferase 2

locus: BSU_02230

pI: 4.72

mw: 41.93 kDa

protein length: 384 aa

gene length: 1155 bp

function: purine biosynthesis

product: phosphoribosylglycinamide formyltransferase 2

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.2|Biosynthesis/ acquisition of nucleotides] → [category|SW 2.5.2.1|Biosynthesis/ acquisition of purine nucleotides]

Gene

Coordinates: 243,892 → 245,046

The protein

Catalyzed reaction/ biological activity

• Formate + ATP + 5'-phospho-ribosylglycinamide = 5'-phosphoribosyl-N-formylglycinamide + ADP + diphosphate (according to Swiss-Prot)

Protein family

• PurK/PurT family (with [protein|0DA73E4D1935F6A91BE251EF2704A11DE5D81174|PurK], according to UniProt)

Paralogous protein(s)

• [protein|0DA73E4D1935F6A91BE251EF2704A11DE5D81174|PurK]

Structure

• [PDB|1KJ9] (with Mg-ATP from ''Escherichia coli'', 55% identity, 69% similarity) [Pubmed|11953435]

Expression and Regulation

Operons

• genes: [gene|C00E34EF63B5EAE33CAD5E469893C0A5112B9F35|purT]
  description: [Pubmed|22383849]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|7496533], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

Biological materials

Mutant

• BKE02230 (Δ[gene|C00E34EF63B5EAE33CAD5E469893C0A5112B9F35|purT]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE02230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTGCCCCTCCTATCT, downstream forward: _UP4_TAGAGTTTGAACAGGTCTTG
• BKK02230 (Δ[gene|C00E34EF63B5EAE33CAD5E469893C0A5112B9F35|purT]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK02230 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTTGCCCCTCCTATCT, downstream forward: _UP4_TAGAGTTTGAACAGGTCTTG

References

• 7496533