purT

phosphoribosylglycinamide formyltransferase 2

Locus: BSU_02230

Isoelectric point: 4.72

Molecular weight: 41.93 kDa

Protein length: 384 aa

Gene length: 1155 bp

Function: purine biosynthesis

Product: phosphoribosylglycinamide formyltransferase 2

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Nucleotide metabolism → Biosynthesis/ acquisition of nucleotides → Biosynthesis/ acquisition of purine nucleotides

Gene

Coordinates: 243,892 → 245,046

The protein

Catalyzed reaction/ biological activity

• ATP + formate + N¹-(5-phospho-D-ribosyl)glycinamide --> ADP + H⁺ + N²-formyl-N¹-(5-phospho-D-ribosyl)glycinamide + phosphate (according to UniProt)

Protein family

• PurK/PurT family (with PurK, according to UniProt)

Paralogous protein(s)

• PurK

Domains

• ATP-grasp domain (aa 111-300) (according to UniProt)

Structure

• 1KJ9 (with Mg-ATP from Escherichia coli, 55% identity, 69% similarity) PubMed

Expression and Regulation

Operons

• Genes: purT
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

Biological materials

Mutant

• BKE02230 (ΔpurT::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTTTGCCCCTCCTATCT, downstream forward: _UP4_TAGAGTTTGAACAGGTCTTG
• BKK02230 (ΔpurT::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCTTTGCCCCTCCTATCT, downstream forward: _UP4_TAGAGTTTGAACAGGTCTTG

References

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