ytrB

[SW|ABC transporter] (ATP-binding protein)

locus: BSU_30450

pI: 5.51

mw: 33.67 kDa

protein length: 292 aa

gene length: 879 bp

function: unknown

product: [SW|ABC transporter] (ATP-binding protein)

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.4|Unknown ABC transporters]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.5|Cold stress proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,117,976 → 3,118,854

The protein

Protein family

• [SW|ABC transporter superfamily] (according to UniProt)

[SW|Domains]

• [SW|ABC transporter domain] (aa 2-227) (according to UniProt)

Structure

• [PDB|1VPL] (from Thermotoga maritima, 31% identity)

[SW|Localization]

• cell membrane (via [protein|31977FDC549E45911C333623DCA3D8941A93D8FF|YtrC]-[protein|9C8233C48733A1E9271EE7AA1283EA9F446CF9DA|YtrD]) [Pubmed|10986249]

Expression and Regulation

Operons

• genes: [gene|DCFA6025F7B4D7C5F6FB8107DDA6538CED33084D|ytrG]-[gene|6A074CE9AD98A2751A216A2EA8156ACE6FFC7280|ytrA]-[gene|BF81AD95FE6CF7662D012EF293C769E80C093D34|ytrB]-[gene|31977FDC549E45911C333623DCA3D8941A93D8FF|ytrC]-[gene|9C8233C48733A1E9271EE7AA1283EA9F446CF9DA|ytrD]-[gene|4FE6A51A0D9BBBF574EA2EF18B95B5BFC0A64CCB|ytrE]-[gene|9024AB63030C1934A954EE6A378CADBDC977E863|ytrF]
  description: [Pubmed|10986249,21856850]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|10986249,21856850], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|6A074CE9AD98A2751A216A2EA8156ACE6FFC7280|YtrA]: repression, [Pubmed|10986249,21856850], in [regulon|6A074CE9AD98A2751A216A2EA8156ACE6FFC7280|YtrA regulon]

  regulation

  • expressed early in the stationary phase [Pubmed|10986249]

Biological materials

Mutant

• GP3188 (Δ[gene|BF81AD95FE6CF7662D012EF293C769E80C093D34|ytrB]), available in [SW|Jörg Stülke]'s lab
• GP2646 Δ([gene|DCFA6025F7B4D7C5F6FB8107DDA6538CED33084D|ytrG]-[gene|6A074CE9AD98A2751A216A2EA8156ACE6FFC7280|ytrA]-[gene|BF81AD95FE6CF7662D012EF293C769E80C093D34|ytrB]-[gene|31977FDC549E45911C333623DCA3D8941A93D8FF|ytrC]-[gene|9C8233C48733A1E9271EE7AA1283EA9F446CF9DA|ytrD]-[gene|4FE6A51A0D9BBBF574EA2EF18B95B5BFC0A64CCB|ytrE]-[gene|9024AB63030C1934A954EE6A378CADBDC977E863|ytrF])::''ermC'', available in [SW|Jörg Stülke]'s lab
• MGNA-A131 (ytrB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/131 NBRP B. subtilis, Japan]
• BKE30450 (Δ[gene|BF81AD95FE6CF7662D012EF293C769E80C093D34|ytrB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30450 BGSC] and in [SW|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_GTTCAATCATTTTTTTTGCC, downstream forward: _UP4_AGATGGTAGACCGAGGTCTC
• BKK30450 (Δ[gene|BF81AD95FE6CF7662D012EF293C769E80C093D34|ytrB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30450 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GTTCAATCATTTTTTTTGCC, downstream forward: _UP4_AGATGGTAGACCGAGGTCTC

References

• 10986249,10092453,12399512