hxlA [2019-06-19 09:31:40]

3-hexulose-6-phosphate synthase

locus: BSU03460

pI: 4.56

mw: 22.43 kDa

protein length: 210 aa

gene length: 633 bp

function: ribulose monophosphate pathway for formaldehyde fixation

product: 3-hexulose-6-phosphate synthase

essential: no

ec: 4.1.2.43

synonyms: yckG

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.8|Resistance against oxidative and electrophile stress]

Gene

Coordinates: 375,166 → 375,798

The protein

Catalyzed reaction/ biological activity

• D-arabino-hex-3-ulose 6-phosphate = D-ribulose 5-phosphate + formaldehyde (according to Swiss-Prot)

Protein family

• HPS subfamily (according to Swiss-Prot)

Structure

• [PDB|3F4W] (from Salmonella typhimurium, 44% identity)

Expression and Regulation

Operons

• genes: [gene|BF480721B89661BFFE6632BB10C5F4DC29951784|hxlA]-[gene|FB0CEA291CA4EC5FB8410050E61B89EDBA1771F9|hxlB]
  description: [Pubmed|10572115]

  regulatory mechanism

  • [protein|D9A187961CFB9496A6712E9E48AAD384357A3E1C|HxlR]: activation, [Pubmed|10572115], in [regulon|D9A187961CFB9496A6712E9E48AAD384357A3E1C|HxlR regulon]

  regulation

  • induction by formaldehyde ([protein|search|HxlR]) [Pubmed|10572115]

Biological materials

Mutant

• MGNA-C057 (yckG::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2055 NBRP B. subtilis, Japan]
• BKE03460 (Δ[gene|BF480721B89661BFFE6632BB10C5F4DC29951784|hxlA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE03460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTTGATCCACTCCCTT, downstream forward: _UP4_CTGATTGTCCAAGGATAACT
• BKK03460 (Δ[gene|BF480721B89661BFFE6632BB10C5F4DC29951784|hxlA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK03460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGTTGATCCACTCCCTT, downstream forward: _UP4_CTGATTGTCCAAGGATAACT

References

• 10572115,16428816,19170879