rhgR

transcriptional activator (AraC family) of the rhamnogalacturonan operon

Locus: BSU_07010

Isoelectric point: 7.77

Molecular weight: 87.56 kDa

Protein length: 761 aa

Gene length: 2286 bp

Function: regulation of pectin utilization

Product: transcriptional activator of the rhamnogalacturonan operon

Essential: no

Synonyms: yesS

Genomic Context

Loading

Categories containing this gene/protein

• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of pectin
• Information processing → Regulation of gene expression → Transcription factors and their control → Transcription factors/ other
• Groups of genes → Membrane proteins

Gene

Coordinates: 765,838 → 768,123

The protein

Protein family

• AraC family

Domains

• HPr-binding domain: aa 406 - 510 PubMed
• HTH araC/xylS-type domain (aa 659-756) (according to UniProt)

Effectors of protein activity

• interaction with HPr(His)-P]] (occurs in the absence of preferred carbon sources) stimulates YesS activity PubMed

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: rhgR-yesT-yesU-yesV-yesW-yesX-yesY-yesZ
  Description: PubMed

  Regulatory mechanism

  • RhgR: activation, PubMed, in RhgR regulon

  Regulation

  • induced by pectin (RhgR) PubMed

Biological materials

Mutant

• MGNA-B450 (yesS::erm), available at the NBRP B. subtilis, Japan
• BKE07010 (ΔrhgR::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCCTCTCCCCCCGCCGC, downstream forward: _UP4_GAAACGCCGTGAAAGGAGAG
• BKK07010 (ΔrhgR::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCCCTCTCCCCCCGCCGC, downstream forward: _UP4_GAAACGCCGTGAAAGGAGAG

References

Loading