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yocH [2019-12-09 11:59:56]
peptidoglycan hydrolase (amidase)
Molecular weight
30.03 kDa
Function
cell wall turnover
Product
peptidoglycan hydrolase
Genomic Context
Categories containing this gene/protein
Gene
Coordinates
2,092,899 → 2,093,762
The protein
Expression and Regulation
Operons
Sigma factors
Regulatory mechanism
Regulation
expression is modulated in response to D,L-endopeptidase activity (increased upon reduced activity in the absence of CwlO or FtsE-FtsX, decreased upon overexpression of LytE) (WalR) PubMedexpressed under conditions that trigger sporulation (Spo0A) PubMed view in new tabBiological materials
Mutant
MGNA-A321 (yocH::erm), available at the NBRP B. subtilis, JapanAH023 knock-out mutant (kan), available in Kevin Devine's and Jörg Stülke's labs PubMedBKE19210 (yocH::erm, available in the BGSC and in Jörg Stülke's lab) PubMedBKE19210 (ΔyocH::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAAGTAAATCCTCCCTTG, downstream forward: _UP4_TAATAATATAGATTGTGATABKK19210 (ΔyocH::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAAGTAAATCCTCCCTTG, downstream forward: _UP4_TAATAATATAGATTGTGATA Expression vectors
pGP1691, expression of yocH-Strep in B. subtilis, in pGP382, available in Jörg Stülke's labpGP1693, expression of yocH-Strep in E. coli, in pGP574, available in Jörg Stülke's lab Labs working on this gene/protein
References
Reviews
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Original publications
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