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yocH [Tue Dec 13 2016 11:16:33 GMT+0100 (CET)]
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yocH [Tue Dec 13 2016 11:16:33 GMT+0100 (CET)]

peptidoglycan hydrolase (amidase)
locus
BSU19210
pI
8.00
mw
30.00 kDa
function
cell wall turnover
product
peptidoglycan hydrolase
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.3|Cell wall degradation/ turnover] → [category|SW 1.1.3.1|Autolysis]
  • [category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins]

    • [SW|Categories] containing this gene/protein

  • [SW|cell wall degradation/ turnover]

    • This gene is a member of the following [SW|regulons]

  • [SW|AbrB regulon], [SW|Spo0A regulon], [SW|WalR regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    2,092,899 -> 2,093,762

    The protein

    [SW|Domains]

  • contains two N-acetylglucosamine-polymer-binding [SW|LysM domain]s [Pubmed|18430080]

    • [SW|Localization]

  • extracellular (signal peptide) [Pubmed|18957862]

    • Expression and Regulation

    Operon

  • ''yocH'' [Pubmed|12950927]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|12950927]

    • Regulation

  • expressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]
  • repressed during logrithmic growth ([protein|search|AbrB]) [Pubmed|15101989]
  • induced in response to cell wall derived muropeptides derived from growing cells but not lysed cells (requires activities of [protein|search|PrkC] and [protein|search|YocH]) [Pubmed|20070526]

    • Regulatory mechanism

  • [SW|Spo0A]: transcription activation [Pubmed|14651647]
  • [protein|search|AbrB]: transcription repression [Pubmed|15101989]
  • [protein|search|WalR]: transcription activation [Pubmed|12950927]

    • Biological materials

    Mutant

  • AH023 knock-out mutant (kan), available in [SW|Kevin Devine]'s and [SW|Jörg Stülke]'s labs [Pubmed|17581128]
  • BKE19210 (''[protein|search|yocH]''::''erm'', available in the BGSC and in [SW|Jörg Stülke]'s lab)

    • Expression vector

  • pGP1691, expression of ''yocH''-Strep in ''B. subtilis'', in [SW|pGP382], available in [SW|Jörg Stülke]'s lab
  • pGP1693, expression of ''yocH''-Strep in ''E. coli'', in [SW|pGP574], available in [SW|Jörg Stülke]'s lab

    • Labs working on this gene/protein

  • [SW|Kevin Devine]

    • References

    Reviews

  • 18430080

    • Original publications

  • 14651647,15101989,18957862,12950927,20059685,20070526,23199363,17581128