yocH [Tue Dec 13 2016 11:16:33 GMT+0100 (CET)]

peptidoglycan hydrolase (amidase)

Locus: BSU19210

Isoelectric point: 8.00

Molecular weight: 30.00 kDa

Function: cell wall turnover

Product: peptidoglycan hydrolase

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Cell envelope and cell division → Cell wall degradation/ turnover → Autolysis
• Groups of genes → Secreted proteins

Categories containing this gene/protein

• cell wall degradation/ turnover

This gene is a member of the following regulons

• AbrB regulon, Spo0A regulon, WalR regulon

Gene

Coordinates on the chromosome (coding sequence): 2,092,899 -> 2,093,762

The protein

Domains

• contains two N-acetylglucosamine-polymer-binding LysM domains PubMed

Localization

• extracellular (signal peptide) PubMed

Expression and Regulation

Operon

• yocH PubMed

Sigma factor

• SigA PubMed

Regulation

• expressed under conditions that trigger sporulation (Spo0A) PubMed
• repressed during logrithmic growth (AbrB) PubMed
• induced in response to cell wall derived muropeptides derived from growing cells but not lysed cells (requires activities of PrkC and YocH) PubMed

Regulatory mechanism

• Spo0A: transcription activation PubMed
• AbrB: transcription repression PubMed
• WalR: transcription activation PubMed

Biological materials

Mutant

• AH023 knock-out mutant (kan), available in Kevin Devine's and Jörg Stülke's labs PubMed
• BKE19210 (yocH::erm, available in the BGSC and in Jörg Stülke's lab)

Expression vector

• pGP1691, expression of yocH-Strep in B. subtilis, in pGP382, available in Jörg Stülke's lab
• pGP1693, expression of yocH-Strep in E. coli, in pGP574, available in Jörg Stülke's lab

Labs working on this gene/protein

• Kevin Devine

References

Reviews

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Original publications

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