chemoreceptor deaminase, required for methylation of methyl-accepting chemotaxis proteins by CheR, enhances phosphatase activity of CheC, required for full McpC activity
function
motility and chemotaxis
product
protein deaminase, CheC activity modulator
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.7|Protein deaminase][category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.1|Motility and chemotaxis] → [category|SW 4.1.1.1|Signal transduction in motility and chemotaxis] → [category|SW 4.1.1.1.1|Soluble signalling proteins][category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.6|Other proteins required for efficient pellicle biofilm formation][SW|Categories] containing this gene/protein
[SW|protein modification], [SW|motility and chemotaxis]This gene is a member of the following [SW|regulons]
[SW|CodY regulon], [SW|DegU regulon], [SW|SigD regulon], [SW|Spo0A regulon]Gene
Phenotypes of a mutant
not essential for pellicle biofilm formation, but mutant is outcompeted by the wild-type strain when competed during pellicle formation [Pubmed|26122431]The protein
Catalyzed reaction/ biological activity
binds chemoreceptors and increases their ability to activate the kinase activity of [protein|search|CheA] [Pubmed|23226535]Protein L-glutamine + H2O = protein L-glutamate + NH3 (according to Swiss-Prot)deaminates Gln-586, Gln-593, and Gln594 in [protein|search|McpA] [Pubmed|22931217]deaminates Gln-609 in [protein|search|McpC] [Pubmed|22931217]Protein family
cheD family (according to Swiss-Prot)[SW|Interactions]
[protein|search|CheC]-[protein|search|CheD] [Pubmed|17908686], this interaction is enhanced by [protein|search|CheY]-P [Pubmed|23226535][protein|search|CheD]-[protein|search|McpA], deamination of Gln-586, Gln-593 and Gln-594 in [protein|search|McpA] [Pubmed|22931217][protein|search|CheD]-[protein|search|McpC], deamination of Gln-609 in [protein|search|McpC] [Pubmed|22931217][protein|search|McpB]-[protein|search|CheD] to increase the ability of [protein|search|McpB] to activate [protein|search|CheA] [Pubmed|25039821]Expression and Regulation
Operon
part of the [[fla-che operon]]''[protein|search|flgB]-[protein|search|flgC]-[protein|search|fliE]-[protein|search|fliF]-[protein|search|fliG]-[protein|search|fliH]-[protein|search|fliI]-[protein|search|fliJ]-[protein|search|ylxF]-[protein|search|fliK]-[protein|search|flgD]-[protein|search|flgE]-[protein|search|fliL]-[protein|search|fliM]-[protein|search|fliY]-[protein|search|cheY]-[protein|search|fliZ]-[protein|search|fliP]-[protein|search|fliQ]-[protein|search|fliR]-[protein|search|flhB]-[protein|search|flhA]-[protein|search|flhF]-[protein|search|flhG]-[protein|search|cheB]-[protein|search|cheA]-[protein|search|cheW]-[protein|search|cheC]-[protein|search|cheD]-[protein|search|sigD]-[protein|search|swrB]'' [Pubmed|9657996,8157612,15175317]''[protein|search|ylxF]-[protein|search|fliK]-[protein|search|flgD]-[protein|search|flgE]-[protein|search|fliL]-[protein|search|fliM]-[protein|search|fliY]-[protein|search|cheY]-[protein|search|fliZ]-[protein|search|fliP]-[protein|search|fliQ]-[protein|search|fliR]-[protein|search|flhB]-[protein|search|flhA]-[protein|search|flhF]-[protein|search|flhG]-[protein|search|cheB]-[protein|search|cheA]-[protein|search|cheW]-[protein|search|cheC]-[protein|search|cheD]-[protein|search|sigD]-[protein|search|swrB]'' [Pubmed|20233303][SW|Sigma factor]
''[protein|search|flgB]'': [protein|search|SigA] [Pubmed|9657996], [protein|search|SigD] [Pubmed|9657996]''[protein|search|ylxF]'': [protein|search|SigD] [Pubmed|20233303]Regulation
see [[fla-che operon]]Regulatory mechanism
see [[fla-che operon]]Biological materials
Mutant
1A861 ( ''cheD''::''cat''), [Pubmed|7893679], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A861&Search=1A861 BGSC]1A863 ( ''cheD''::''cat''), [Pubmed|7893679], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A863&Search=1A863 BGSC]DS6868 (marker-less in NCIB3610) [Pubmed|25313396]References
Reviews
Original publications
25313396,26122431,17850253,21515776,22931217,23226535,7893679,15317802,17908686,12011078,8866475,11722727,9194713,14651647,9657996,8157612,15175317,16469702,24386445,25039821 
